世界针灸杂志（英文版）2025，Vol.35Issue(2)：125-134,10.DOI:10.1016/j.wjam.2025.02.003
基于PI3K/AKT/mTOR信号通路探究不同温度艾灸对压疮大鼠创面愈合及细胞凋亡的影响
Effects of moxibustion at different temperature on wound healing and apoptosis in rats with pressure ulcer based on PI3K/AKT/mTOR signal pathway☆
摘要
Abstract
Objective:To investigate the effects of moxibustion on wound healing and evaluate whether the efficacy of moxibustion at different temperatures varies.Additionally,to explore the mechanism by which moxi-bustion regulates the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammalian rapamycin target protein(mTOR)signaling pathway on wound healing and cell apoptosis. Methods:A total of 90 SPF-grade SD rats were randomly divided into six groups(15 rats per group):control group,model group,recombinant bovine basic fibroblast growth factor gel(rb-bFGF)group,mox-ibustion group A[temperature(42±1)℃],moxibustion group B[temperature(45±1)℃],and moxi-bustion group C[temperature(48±1)℃].Except the control group,the interventions were conducted in the other groups with pressure ulcer induced by ischemia/reperfusion injury.In the control group,povidone-iodine was administered at the knee joint.In the model group,after povidone-iodine applied at the pressure ulcer,petroleum jelly gauze was bandaged.In the rb-bFGF group,after povidone-iodine applied at the pressure ulcer,rb-bFGF was smeared and petroleum jelly gauze was bandaged.In the moxibustion group A,the suspending moxibustion with moxa stick was delivered 3 cm to 5 cm above the ulcer.The paperless temperature recorder probe was placed on the wound surface where moxibus-tion was delivered,and the real-time temperature was monitored and adjusted,kept at(42±1)℃.The moxibustion temperature was kept at(45±1)℃ in the moxibustion group B,and(48±1)℃ in the moxibustion group C.The intervention lasted 14 days in each group.The wound healing rates were com-pared on the 3,5,7 and 14 days after the first intervention among the 6 groups.After 14-day intervention respectively,the histopathological changes were observed with HE staining,apoptosis was detected with TUNEL method and flow cytometry;VEGF-A protein expression determined with immunohistochemistry;the contents of Interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and transforming growth factor-β(TGF-β)in serum with ELISA,and PI3K/AKT/mTOR related protein and mRNA expression was with West-ern blotting and qRT-PCR in wound tissues of each group. Results:Regarding the intervention effects,(1)compared with the control group,the model group showed significant inflammatory infiltration in wound tissues,higher levels of cell apoptosis,and ele-vated serum levels of IL-6,TNF-α and TGF-β(P＜0.05);(2)compared with the model group,the rb-bFGF group and 3 moxibustion groups showed higher wound healing rates(P＜0.05),reduced inflammatory infiltration in the wound tissues,lower levels of cell apoptosis,and decreased serum levels of IL-6,TNF-α and TGF-β(P＜0.05).Among the moxibustion groups,group C showed significantly better results than group A(P＜0.01).Regarding the mechanism,(1)compared with the control group,the model group exhibited significantly higher protein and mRNA expression levels of PI3K/AKT/mTOR signal path-way components(P＜0.05);(2)compared with the model group,the rb-bFGF group and 3 moxibustion groups showed lower protein and mRNA expression levels of PI3K/AKT/mTOR signal pathway components(P＜0.05).Among the moxibustion groups,group C showed significantly higher expression levels than group A(P＜0.01). Conclusion:Moxibustion significantly promotes wound healing,with high-temperature moxibustion being more effective than low-temperature moxibustion in reducing the expression of inflammatory factors and cell apoptosis,thereby improving wound healing rates.It is presented that the higher moxibustion tem-perature,the higher wound healing rate,which may be associated with the regulation of PI3K/AKT/mTOR signal pathway with moxibustion.关键词
Pressure ulcer/Moxibustion warming/PI3K/AKT/mTOR/Apoptosis/Inflammatory factorKey words
Pressure ulcer/Moxibustion warming/PI3K/AKT/mTOR/Apoptosis/Inflammatory factor引用本文复制引用
潘虹,葸英博,丁劲,尹秦,薛慧琴,岳海玉..基于PI3K/AKT/mTOR信号通路探究不同温度艾灸对压疮大鼠创面愈合及细胞凋亡的影响[J].世界针灸杂志（英文版）,2025,35(2):125-134,10.基金项目
Supported by Gansu Youth Science and Technology Fund Project:20JR10RA344. （）
