西华大学学报（自然科学版）2025，Vol.44Issue(4)：1-10,10.DOI:10.12198/j.issn.1673-159X.5518
肉苁蓉提取物对地塞米松诱导小鼠肌少症的治疗效果及作用机制研究
Study on the Therapeutic Effects and Mechanisms of Cistanche Deserticola Extract on Dexamethasone-Induced Sarcopenia in Mice
摘要
Abstract
This study explored the therapeutic effect of Cistanche deserticola extract(CdE)on dexa-methasone(Dex)-induced sarcopenia in mice and the possible mechanism of action.Firstly,the pharmaco-dynamic components and important targets of Cistanche were studied by network pharmacology,and the pathways were enriched to predict the mechanism of action of CdE.In animal experiments,50 KM mice were randomly divided into 5 groups(control group,Dex model group,CdEhigh-dose group,CdE medium-dose group,and CdE low-dose group).Except for the control group,the other groups were injected subcu-taneously to induce sarcopenia after 42 days,and the three treatment groups were given different doses of CdE orally at the same time.During the experiment,the animal weight,exercise capacity and body compos-ition were regularly tested.After the animals were sacrificed,the muscle coefficient of hind limbs was cal-culated,and histology and related gene expression tests were performed.Network pharmacology results show that CdE may treat sarcopenia by upregulating IGF-1-re-lated signaling pathways.The results of an-imal experiments show that CdE significantly reverse the decrease of lean meat content and muscle coeffi-cient caused by Dex,improve exercise capacity,reduce fat content,improve the histological status of the muscles,up-regulated IGF-1,MyoD and MyoG,and down-regulated the expression of Atrogin-1 and MuRF-1.CdE has a therapeutic effect on Dex-induced muscle atrophy and can improve muscle histologic-al morphology,and its mechanism of action may be the activation of IGF-1 signaling pathway.关键词
肉苁蓉提取物/地塞米松/肌少症/网络药理学/IGF-1通路Key words
Cistanche deserticola extract/dexamethasone/sarcopenia/network pharmacology/IGF-1 pathway分类
医药卫生引用本文复制引用
方雅静,杨熙,李昕怡,叶明珠,刘丹,赖朋..肉苁蓉提取物对地塞米松诱导小鼠肌少症的治疗效果及作用机制研究[J].西华大学学报（自然科学版）,2025,44(4):1-10,10.基金项目
教育部春晖计划项目(14205506). （14205506）
