上海交通大学学报（医学版）2025，Vol.45Issue(5)：562-569,8.DOI:10.3969/j.issn.1674-8115.2025.05.004
Nd:YAP激光生物刺激通过WNT/β-catenin信号通路促进人牙周韧带细胞增殖和成骨分化
Promotion of Nd:YAP laser biostimulation on the proliferation and osteogenic differentiation of human periodontal ligament cells through WNT/β-catenin signaling pathway
摘要
Abstract
Objective·To study the effect of neodymium-doped yttrium aluminum perovskite(Nd:YAP)laser biostimulation on the proliferation and osteogenic differentiation of human periodontal ligament cells(hPDLCs)and its possible mechanism.Methods·Five premolars removed for orthodontic reasons were collected from Changzhou Stomatological Hospital,and the periodontal ligament tissues from the middle 1/3 of the roots were taken to culture hPDLCs in vitro.The cells were irradiated with the biostimulation function[G(-)mode]of the Nd:YAP laser.According to the irradiation time,the cells were divided into a control group(without laser irradiation),and groups irradiated for 5 s,10 s,15 s,20 s and 30 s.The cell counting kit-8(CCK-8)method was used to detect the proliferation of hPDLCs in each group.After osteogenic differentiation was induced,the alkaline phosphatase(ALP)content and activity level of the cells were detected using an ALP staining kit and an ALP activity detection kit.The calcium salt level of the cells was evaluated by alizarin red S staining and calcium quantitative analysis.The expression of genes and proteins related to the WNT/β-catenin signaling pathway,including dickkopf-related protein 1(DKK-1),β-catenin,and runt-related transcription factor 2(RUNX2),was analyzed by quantitative real-time PCR(qPCR)and Western blotting.Results·The results of CCK-8 showed that the proliferation level of cells in the 10 s,15 s,20 s,and 30 s groups was enhanced from 3 d after irradiation(all P<0.05).After induction of osteogenic differentiation,ALP content,activity,and calcium salt level in the laser irradiation groups increased with the extension of irradiation time(all P<0.05).The results of qPCR and Western blotting analysis showed that the expression levels of the DKK-1 gene and protein in the laser irradiation groups decreased with the extension of irradiation time.However,the expression levels of β-catenin and RUNX2 genes and proteins increased significantly with the extension of irradiation time;there were statistically significant differences between the 15 s,20 s,and 30 s groups and the control group(all P<0.05).Conclusion·Nd:YAP laser biostimulation may promote proliferation and osteogenic differentiation of hPDLCs through WNT/β-catenin signaling pathway.关键词
掺钕钇铝钙钛矿激光/激光生物刺激效应/人牙周韧带细胞/细胞增殖/成骨分化Key words
neodymium-doped yttrium aluminum perovskite laser(Nd:YAP laser)/laser biostimulation effect/human periodontal ligament cell/cell proliferation/osteogenic differentiation分类
医药卫生引用本文复制引用
许沐馨,刘贤,蒋立姗,孙青..Nd:YAP激光生物刺激通过WNT/β-catenin信号通路促进人牙周韧带细胞增殖和成骨分化[J].上海交通大学学报（医学版）,2025,45(5):562-569,8.基金项目
常州市卫生健康委员会重大科技项目(ZD202221). Major Science and Technology Project of Changzhou Health Commission(ZD202221). （ZD202221）
