上海交通大学学报（医学版）2025，Vol.45Issue(5)：605-613,9.DOI:10.3969/j.issn.1674-8115.2025.05.009
两样本孟德尔随机化分析血浆磷脂酰乙醇胺水平与结直肠腺癌发病风险的关系
Causal association between plasma phosphatidylethanolamine and risk of colorectal adenocarcinoma:a two-sample Mendelian randomization study
摘要
Abstract
Objective·To employ the two-sample Mendelian randomization(TSMR)method,using genetic variants as instrumental variables,to investigate the causal relationship between phosphatidylethanolamine(PE)and the risk of colorectal adenocarcinoma.Methods·The single-nucleotide polymorphism(SNP)data associated with PE and colorectal adenocarcinoma were obtained from the Medical Research Council Integrative Epidemiology Unit(MRC IEU)at the University of Bristol and the Finnish Biobank,respectively.A secondary data analysis was conducted using summary statistics from genome-wide association studies(GWAS),and genetic loci strongly associated with PE were selected as instrumental variables.Four Mendelian randomization(MR)methods,inverse-variance weighted(IVW)method,MR-Egger regression,weighted median(WME)method,and weighted mode(WM)method,were employed to assess the causal effect.The IVW method was used as the primary statistical approach,while MR-Egger,WME,and WM served as supplementary methods.Rigorous assessments for robustness included MR-Egger regression,MR-PRESSO global tests for horizontal pleiotropy,and Cochran's Q test to evaluate heterogeneity.Results·Ten instrumental variables were selected,and the Steiger test indicated that all PE-associated SNPs exhibited a consistent direction of causal effect on colorectal cancer.Among the 10 SNPs,rs102275 and rs9393903 showed the strongest positive associations with colorectal adenocarcinoma risk,with effect sizes of 0.45(P=8.01×10-5)and 0.82(P=2.31×10-2),respectively.Consistent findings from MR analyses demonstrated that PE elevated the risk of colorectal adenocarcinoma across all four methods.In the IVW analysis,the OR was 1.36(95%CI 1.17‒1.59,P=7.24×10-5).In the MR-Egger regression,the OR was 1.44(95%CI 0.97‒2.14,P=1.12×10-1).In the WEM analysis,the OR was 1.33(95%CI 1.07‒1.65,P=8.81×10-3).In the WM analysis,the OR was 1.41(95%CI 1.12‒1.77,P=1.70×10-2).Cochran's Q test revealed no heterogeneity among the effect estimates of the 10 SNPs on colorectal adenocarcinoma.Both MR-Egger regression intercept and MR-PRESSO test indicated no evidence of horizontal pleiotropy among the SNPs.Leave-one-out analysis showed overlapping confidence intervals after excluding any single SNP,indicating that the results were not sensitive to individual SNPs and were highly robust.Conclusions·There is a causal association between circulating PE levels and the risk of colorectal adenocarcinoma.A genetically predicted increase of one standard deviation in plasma PE levels is associated with a 1.36-fold higher risk of developing colorectal adenocarcinoma(95%CI 1.17‒1.59,P=7.24×10-5).关键词
结直肠腺癌/磷脂酰乙醇胺/两样本孟德尔随机化/因果关联/全基因组关联研究Key words
colorectal adenocarcinoma/phosphatidylethanolamine/two-sample mendelian randomization/causal association/genome-wide association study分类
医药卫生引用本文复制引用
徐苓,皇甫昱婵,沈立松,马妍慧..两样本孟德尔随机化分析血浆磷脂酰乙醇胺水平与结直肠腺癌发病风险的关系[J].上海交通大学学报（医学版）,2025,45(5):605-613,9.基金项目
上海市科学技术委员会项目(23S31905800). Program of Science and Technology Commission of Shanghai Municipality(23S31905800). （23S31905800）
