塔里木大学学报2025，Vol.37Issue(3)：9-15,7.DOI:10.3969/j.issn.1009-0568.2025.03.002
喀什地区某引种场奶牛芽囊原虫感染情况及其亚型鉴定
Infection status and molecular characterization of Blastocystis in cattle in an imported farm in Kashgar
摘要
Abstract
To investigate the infection status and subtype distribution of Blastocystis in cows within an import farm in Kashgar,Xinjiang Uygur Autonomous region,a total of 186 fresh fecal samples were collected and extracted genomic DNA.Specimens were screened for the presence of Blastocystis via PCR based on the small subunit ribosomal RNA(SSU rRNA)gene,followed by sequencing of positive amplicons for subtype identification.Sequence assembly and phylogenetic reconstruction were performed using bioinformatics tools.According to PCR analyses,overall prevalence of Blastocystis was determined as 40.3%(75/186),with the highest positive rate(96.0%,48/50)observed in samples aged 6-12 months.The sequence analyses confirmed that a total of 7 known livestock-specific subtypes:ST10,ST14,ST24,ST25,ST26,ST42 and ST44,among which ST42 emerged as the predominant subtype.The imported farm exhibited a high prevalence of Blastocystis infection with demonstrated genetic diversity.It was underscore that the pathogen should not be neglected,preventive measures should be taken in this area to prevent the transmission risk of Blastocystis.关键词
新疆/芽囊原虫/奶牛/感染率/亚型鉴定Key words
Xinjiang/Blastocystis spp./dairy cattle/infection rate/subtype identification分类
畜牧业引用本文复制引用
赵慧钢,郭辰玉,蔡雯,朱亚楠,啜力文,齐萌,余复昌..喀什地区某引种场奶牛芽囊原虫感染情况及其亚型鉴定[J].塔里木大学学报,2025,37(3):9-15,7.基金项目
塔里木大学校长基金博士人才项目(TDZKBS202208) （TDZKBS202208）
国家级大学生创新创业训练计划项目(202310757004) （202310757004）
2022年度兵团指导性科技计划项目(2022ZD123) （2022ZD123）
新疆维吾尔自治区"天池英才"引进计划项目 （）
