现代纺织技术2025，Vol.33Issue(6)：91-99,9.DOI:10.19398/j.att.202407018
环糊精包合大蒜素在普鲁兰/聚乙烯醇纳米纤维膜负载及其抗菌性能
Loading and antimicrobial properties of cyclodextrin-encapsulated allicin in pullulan/polyvinyl alcohol nanofiber membranes
摘要
Abstract
Due to the instability,irritating odor and non-electrospinning of Alli,its application in the food packaging industry has been limited to a certain extent.In this paper,biodegradable biobase is used as a film-forming material loaded with Alli inclusion,to improve the defects of Alli and enhance the value of Alli utilization.Currently,the preparation of biodegradable antimicrobial nanofiber membranes has become an important research direction in the food packaging industry.Alli has a spectral antibacterial effect and cyclodextrin(β-CD)has a hydrophobic cavity structure and is non-toxic.Alli/β-CD-IC was obtained by wrapping Alli molecules in β-CD molecules by rotary evaporation.Alli/β-CD-IC(1∶3)with mass fractions of 2％,4％and 6％were added to the PUL/PVA hybrid spinning solution,and electrostatic spinning was utilized to obtain PUL/PVA@Alli/β-CD-IC nanofiber membranes under a voltage 20 kV,flow rate of 0.4 mL/h,receiving distance of 13 cm,temperature of 23 ℃,and relative humidity of 38％. Analyzing the scanning electron microscope images and observing the Alli/β-CD-IC images all showed irregular surfaces,which were different from the morphology presented by the pure β-CD,and the original morphology was significantly changed.In the PUL/PVA(7∶3)mixture,there are smaller beads in the PUL/PVA@Alli/β-CD-IC nanofiber membrane,and the nanofiber membrane prepared by adding Alli/β-CD-IC has better morphology and uniform fiber diameter,which indicates that PUL/PVA@Alli/β-CD-IC nanofibers have a filamentous and continuous fiber structure.The analysis of infrared spectra and the comparison of PUL/PVA hybrid nanofiber membranes with PUL and PVA as-received fiber membranes showed that the O-H bond vibration peak was shifted,and there was intermolecular interaction between PVA and PUL.The spectra of the characteristic peaks of PUL/PVA nanofibrous membranes loaded with Alli/β-CD-IC were like those of PUL/PVA nanofibrous membranes,while the Alli/β-CD-IC characteristic peak disappeared.The XRD results showed that the peaks of PUL/PVA nanofiber membranes were shifted to the right,indicating that the close interaction between PUL and PVA molecules hindered the formation of a crystal structure,and the formation of hydrogen bonds through the interaction of PUL and PVA polymers resulted in the intermolecular interaction force that would act differently with different proportions of PVA.The peaks of the nanofiber membranes loaded with Alli/β-CD-IC were all shifted to the right and did not show any special peaks like those of Alli/β-CD-IC and sharp peaks of the crystal structure,suggesting intermolecular interactions that hindered the formation of crystals and led to an amorphous structure.The number of colonies on the solid medium was significantly reduced when 0.8 g of Alli/β-CD-IC was added in comparison,and the inhibition rate could reach 99.82％±0.17％;the PUL/PVA@Alli/β-CD-IC nanofiber membrane prepared with loaded Alli/β-CD-IC had a significant inhibitory property on the growth of E.coli,and the inhibition rate could reach 99.91％±0.07％.The experimental results showed that allicin and its inclusion complexes had significant growth inhibition effect on fungi. Expanding the field of food packaging to prepare green and degradable antimicrobial nanomaterials,PUL and PVA were used as spinning substrates,loaded with Alli/β-CD-IC,and PUL/PVA@Alli/β-CD-IC nanofibrous membranes were prepared under certain spinning conditions,and the results are shown below.In this paper,in order to improve the Alli instability and unpleasant odor,Alli and β-CD were encapsulated with a mass ratio of 1∶3,and the encapsulation efficiency reached 98.6％.The formed Alli/β-CD-IC still had a significant antimicrobial effect,and the inhibition rate could reach 99.82％.PUL and PVA were selected as the substrate membranes and loaded with Alli/β-CD-IC to improve the utilization of Alli/β-CD-IC,and the experiments proved that the degradable substrate membranes were loaded with Alli/β-CD-IC,and PUL/PVA@Alli/β-CD-IC nanofibrous membranes were successfully prepared to improve the application range of Alli.Different ratios of PUL/PVA@Alli/β-CD-IC nanofiber membranes inhibited the growth of E.coli with an antimicrobial efficiency of 99.91％.The degradable nanofiber membranes not only changed the defects of antimicrobial essential oils that were unstable and difficult to load,but also provided a new way of thinking about the application of Alli in different fields.关键词
静电纺/大蒜素/环糊精/纳米纤维膜/包合物/抗菌性Key words
electrostatic spinning/allicin/cyclodextrin/nanofiber membrane/inclusion/antimicrobial properties分类
轻工纺织引用本文复制引用
周林,钱永芳,吕丽华,高原,周兴海..环糊精包合大蒜素在普鲁兰/聚乙烯醇纳米纤维膜负载及其抗菌性能[J].现代纺织技术,2025,33(6):91-99,9.基金项目
辽宁省教育厅面上项目(LJKMZ20220889) （LJKMZ20220889）
