摘要
Abstract
Objective To explore the biocompatibility of hemodialysis circulation pipeline materials,with a focus on evaluating their interaction with human umbilical vein endothelial cells(HUVECs).Methods Through the CCK8 experiment and staining of live and dead cells,the adhesion,growth and cytotoxic reactions of dialysis tubing materials in different parts to HUVECs were observed.Results HUVECs were cultured and proliferated in different pipeline materials,and cells in different groups could adhere well after 24 h,the proliferation rate could all reach more than 94%after 48 h.After culturing for 72 h,a high cell adhesion rate could be observed under a bright-field microscope.The adhesion rates of the six different pipeline materials to HUVECs all reached more than 90%,and the pipeline materials had no significant effect on the proliferation of cells.The six experimental groups were respectively compared with the negative control group.There was no statistically difference between the experimental group and the control group(P>0.05),indicating that the proliferation rate and adhesion rate of cells in the experimental group were consistent with those in the control group.It can be known from the staining results of live and dead cells that the cell survival rate exceeds 90%,with no cytotoxicity,indicating good biocompatibility.Conclusion This study can provide a scientific basis for the optimization of hemodialysis pipeline materials and the development of new materials,and offer theoretical support for improving the therapeutic effect of dialysis and reducing complications.关键词
血液透析循环管路/人脐静脉内皮细胞/细胞毒性/细胞增殖/生物相容性/CCK-8试验Key words
hemodialysis circulation pipeline/human umbilical vein endothelial cells/cytotoxicity/cell proliferation/biocompatibility/CCK-8 test分类
预防医学
