现代畜牧科技Issue(6)：7-9,3.DOI:10.19369/j.cnki.2095-9737.2025.06.002
多重耐药猪源类肺炎克雷伯菌的全基因组测序分析
Whole-genome Sequencing Analysis of Multidrug Resistance in Porcine Klebsiella quasipneumoniae
摘要
Abstract
In order to identify the main pathogens and drug resistance characteristics of a case of swine pneumonia infection in Jilin region,nasal swab samples of pigs with respiratory diseases were collected for bacterial culture.The dominant strain was identified via 16S rRNA gene sequencing,while drug susceptibility was evaluated using the minimum inhibitory concentration(MIC)method.Whole-genome sequencing(WGS)combined with bioinformatics tools was employed to analyze resistance genes and mechanisms.Results demonstrated that the isolated strain,Yan-10,was identified as Klebsiella quasipneumoniae with a maximum homology of 99.86%to reference sequences.Antimicrobial susceptibility testing revealed resistance to antibiotics,including chloramphenicol,quinolones,and cephalosporins.Genomic analysis uncovered 18 resistance genes,predominantly encoding active efflux pumps(9 genes),target site modification(7 genes),reduced membrane permeability(3 genes),and production of inactivating enzymes(1 gene).These findings indicate that Yan-10 is a multidrug-resistant strain,with intricate resistance mechanisms exacerbating challenges in pneumonia management on pig farms.关键词
多重耐药/类肺炎克雷伯菌/全基因组测序Key words
multidrug resistant/Klebsiella quasipneumoniae/Whole-genome sequencing分类
农业科技引用本文复制引用
刘宇洋,刘玉莹,董文龙,沙万里,张东红..多重耐药猪源类肺炎克雷伯菌的全基因组测序分析[J].现代畜牧科技,2025,(6):7-9,3.基金项目
吉林省科技发展计划项目(20220404008NC) （20220404008NC）
