现代畜牧科技Issue(6)：35-37,3.DOI:10.19369/j.cnki.2095-9737.2025.06.010
多重耐药四川克吕沃尔氏菌LHN-g4的全基因组测序分析
The Whole Genome Sequencing Analysis of Multidrug-resistant Kluyvera sichuanensis LHN-g4
摘要
Abstract
This study aims to elucidate the antimicrobial resistance mechanisms of a strain of Kluyvera sichuanensis(K.sichuanensis)(LHN-g4)isolated from swine in Jilin Province,China.The strain was identified as K.sichuanensis through 16S rRNA gene sequencing.Minimum inhibitory concentration(MIC)assays revealed that the strain exhibits resistance to multiple antibiotics,with particularly high resistance levels to β-lactams,aminoglycosides,and macrolides.Whole genome sequencing analysis demonstrated that the strain possesses a 5 096 378 bp chromosome harboring 18 antimicrobial resistance genes,including 8 contiguous efflux pump genes adjacent to resistance determinants,which collectively contribute to the resistant phenotype of LHN-g4.Notably,CTX-M-95,a broad-spectrum β-lactamase,further enhances the strain's resistance profile.Additionally,plasmid N-1 was found to carry genes associated with the type Ⅳ secretion system,two resistance genes,and a composite transposon,all of which participate in the resistance mechanisms of LHN-g4.In conclusion,the antimicrobial resistance of LHN-g4 is determined by the combined effects of chromosomal resistance genes,the type Ⅳ secretion system,and the transposon located on plasmid N-1.关键词
四川克吕沃尔氏菌/CTX-M-95/全基因组测序Key words
Kluyvera sichuanensis/CTX-M-95/whole-genome sequencing分类
医药卫生引用本文复制引用
李涵诺,许诺,杨卓蕾,何颖慧,张广妍,董文龙..多重耐药四川克吕沃尔氏菌LHN-g4的全基因组测序分析[J].现代畜牧科技,2025,(6):35-37,3.基金项目
吉林农业科技学院大学生科技创新创业项目(XJ202411439016) （XJ202411439016）
