空军军医大学学报2025，Vol.46Issue(6)：708-714,7.DOI:10.13276/j.issn.2097-1656.2025.06.002
肺腺癌CD8+T细胞浸润-激活-耗竭预后模型的建立及关键基因ZNF540的作用
Establishment of a prognostic model for CD8+T cell infiltration,activation,and exhaustion in lung adenocarcinoma and role of the key gene ZNF540
摘要
Abstract
Objective To investigate the prognostic significance of CD8+T cell infiltration,activation,and exhaustion in lung adenocarcinoma(LUAD)and analyze the role of key gene.Methods A total of 41 CD8+T cell infiltration-activation-exhaustion genes(TIAEGs)were collected from TCGA and GEO databases.A prognostic model was constructed using LASSO regression,and the accuracy of the model was validated by Kaplan-Meier curves,random forest model,support vector machine model,ROC curves,and Cox regression analysis.The degree of immune cell infiltration was analyzed using the CIBERSORT algorithm.The expression difference of key protein ZNF540 was compared using the HPA database.KMPlotter database and Kaplan-Meier curves were used to analyze the impact of key genes on patient survival and immunotherapy.Results A prognostic model based on 10 TIAEGs was constructed.LUAD patients with higher TIAEGs scores had a poorer prognosis and were negatively correlated with immune cell infiltration.The key gene ZNF540 was significantly down-regulated in LUAD and positively correlated with the degree of immune cell infiltration and enrichment and immunotherapy response.Conclusion The TIAEGs score model and ZNF540 can serve as potential biomarkers for prognosis and immunotherapy response in LUAD.关键词
肺腺癌/CD8+T细胞/免疫浸润/免疫激活/免疫耗竭/预后模型Key words
lung adenocarcinoma/CD8+T cells/immune infiltration/immune activation/immune exhaustion/prognostic model分类
临床医学引用本文复制引用
李妍,马海龙,杜欢,张新宇,高永恒,金发光,潘蕾..肺腺癌CD8+T细胞浸润-激活-耗竭预后模型的建立及关键基因ZNF540的作用[J].空军军医大学学报,2025,46(6):708-714,7.基金项目
陕西省重点研发计划项目(2024SF-YBXM-092) （2024SF-YBXM-092）
