空军军医大学学报2025，Vol.46Issue(6)：715-720,6.DOI:10.13276/j.issn.2097-1656.2025.06.003
特女贞苷通过抑制NF-κB/NLRP3信号通路减轻海水吸入性肺损伤
Protective effects of specnuezhenide on seawater inhalation-induced acute lung injury by inhibiting NF-κB/NLRP3 signaling pathway
摘要
Abstract
Objective To investigate the therapeutic effect of specnuezhenide(SPE)on seawater inhalation-induced acute lung injury(SW-ALI).Methods A total of 24 SPF-grade 8-week-old male C57BL/6J mice were randomly divided into four groups:blank control group(Con group),seawater group(SW group),low-dose SPE-treated group(LD group),and high-dose SPE-treated group(HD group),with 6 mice in each group.LD group and HD group were fed with 50 mg/(kg·d)and 100 mg/(kg·d)SPE by intragastric administration,respectively,while SW group was fed with equal volume of normal saline by intragastric administration.The drug and normal saline were administered once a day for 3 d.The SW-ALI model was established 2 h after the last administration of the drug and normal saline.The mice were executed 24 h after modeling,and the pathological changes of lung tissue were observed by HE staining.The wet-to-dry weight ratio(W/D)of the lungs and the protein content of bronchoalveolar lavage fluid(BALF)of the mice in each group were detected.The levels of IL-6,IL-1β and TNF-α in BALF were detected by ELISA,and qRT-PCR and Western blotting were used to detect NF-κB and NLRP3 mRNA and protein expression levels in lung tissues.Results Compared with Con group,mice in the SW group showed obvious lung injury and increased inflammatory cell infiltration in lung tissues and alveoli.The W/D of the lungs,the protein and IL-6,IL-1β and TNF-α contents in BALF,and the mRNA and protein levels of NF-κB and NLRP3 in lung tissues were all significantly increased(P＜0.05).Compared with SW group,the pathological state of lung tissue in LD group and HD group was significantly alleviated,and the W/D of the lungs,the protein and IL-6,IL-1β and TNF-α contents in BALF,and the mRNA and protein levels of NF-κB and NLRP3 in lung tissues were all significantly reduced(P＜0.05).Conclusion SPE alleviates SW-ALI in mice by inhibiting NF-κB/NLRP3 inflammatory signaling pathway.关键词
特女贞苷/海水吸入性肺损伤/NF-κB/NLRP3Key words
specnuezhenide/seawater inhalation-induced acute lung injury/NF-κB/NLRP3分类
中医学引用本文复制引用
高嘉营,郭显,赵世鸿,王怡沣,金发光..特女贞苷通过抑制NF-κB/NLRP3信号通路减轻海水吸入性肺损伤[J].空军军医大学学报,2025,46(6):715-720,6.基金项目
国家自然科学基金(81970076,82270084) （81970076,82270084）
