中国临床医学2025，Vol.32Issue(3)：493-499,7.DOI:10.12025/j.issn.1008-6358.2025.20241401
麻醉和手术对5×FAD小鼠海马内阿尔茨海默病相关蛋白表达影响及性别差异
Effects of anesthesia and surgery on the expression of Alzheimer's disease-related proteins in the hippocampus of 5×FAD mice and its sex differences
摘要
Abstract
Objective To investigate the impact of anesthesia and surgery on hippocampal expression of Alzheimer's disease(AD)-associated proteins in 5×FAD transgenic mice and explore potential sex differences.Methods 5×FAD mice were crossbred with C57BL/6J wild-type(WT)mice to generate offspring for genotypic confirmation.Four-month-old 5×FAD mice and littermate(LM)WT controls were allocated into 8 experimental groups(n=8/group):female/male 5×FAD control group,female/male 5×FAD anesthesia/surgery group,female/male LM control group,and female/male LM anesthesia/surgery group.Anesthesia/surgery groups underwent laparotomy under 1.4%isoflurane anesthesia,while control groups received no intervention.Hippocampal tissues were collected 24 hours post-procedure for Western blotting analysis of β-catenin,glycogen synthase kinase 3 beta(GSK3β),and phosphorylated GSK3β(p-GSK3β)levels.Results Female 5×FAD mice demonstrated significant reductions in β-catenin levels and p-GSK3β expression compared to both sex-matched LM controls and male 5×FAD counterparts(P＜0.05).No significant differences in these proteins were observed in male 5×FAD mice following anesthesia/surgery.Conclusions These findings reveal sex-specific responses to perioperative stress in AD,suggesting that anesthesia and surgery may affect female AD patients through hippocampal β-catenin/GSK3β pathway modulation.关键词
阿尔茨海默病/麻醉/手术/5×FAD转基因鼠/性别差异/β-catenin/GSK3βKey words
Alzheimer's disease/anesthesia/surgery/5×FAD transgenic mice/sex differences/β-catenin/GSK3β分类
医药卫生引用本文复制引用
张莹林,黄勇,张俪,梁超..麻醉和手术对5×FAD小鼠海马内阿尔茨海默病相关蛋白表达影响及性别差异[J].中国临床医学,2025,32(3):493-499,7.基金项目
国家自然科学基金(82271215).Supported by National Natural Science Foundation of China(82271215). （82271215）
