中国兽医科学2025，Vol.55Issue(6)：797-804,8.DOI:10.16656/j.issn.1673-4696.2025.0106
小反刍兽疫病毒H蛋白B细胞表位的预测与鉴定
Prediction and identification of B cell epitopes on H protein from peste des petits ruminants virus
摘要
Abstract
This study aimed to express the H protein of peste des petits ruminants virus(PPRV)as well as to predict and identify its linear B cell epitopes.The H gene of PPRV was amplified by PCR,and then it was used to construct the recombinant plasmid pET-30a(+)-H.The H protein expressed by the prokaryotic expression system was purified and refolded.Then the refolded H protein was used to immunize mice to prepare polyclonal antiserum.The linear B cell epitopes of H protein were predicted and analyzed by bioinformatics software.The nucleotide sequences of 16 potential epitopes among those predicted were respectively recombined into the pGEX-4T-1 vector.The fusion expression of the predicted epitopes and GST tag protein was accomplished by prokaryotic expression system.The purified fusion proteins of the predicted epitopes were identified by Western-blot and indirect ELISA with the utilization of the prepared polyclonal antiserum.The results showed that the H protein had good immunogenicity.Five linear B cell epitopes were screened and identified by the recombinant H protein polyclonal antibody.This study successfully expressed the H protein of PPRV and predicted and identified five linear B cell epitopes.It provides a reference for the development of diagnostic reagents and epitope vaccines for peste des petits ruminants.关键词
小反刍兽疫病毒/H蛋白/原核表达/线性B细胞表位Key words
peste des petits ruminants virus/H protein/prokaryotic expression/linear B cell epitopes分类
农业科技引用本文复制引用
张石豪,朱真,焦文杰,王诗研,韩凤烨,孙茜,邹兴启,印春生,赵传芳..小反刍兽疫病毒H蛋白B细胞表位的预测与鉴定[J].中国兽医科学,2025,55(6):797-804,8.基金项目
国家重点研发计划项目(2022YFD1800703) （2022YFD1800703）
吉林省科技厅创新平台(基地)和人才专项(20230508009RC) （基地）
