中国兽医科学2025，Vol.55Issue(6)：813-819,7.DOI:10.16656/j.issn.1673-4696.2025.0107
内源性绵羊肺腺瘤病毒LTR转录调控元件的筛选
Screening of transcriptional regulatory elements of endogenous sheep pulmonary adenoma virus LTR
摘要
Abstract
The purpose of this study was to identify the transcriptional regulatory elements of the endogenous jaagsiekte sheep retrovirus(enJSRV)long terminal repeat(LTR).We used homologous recombination technology to construct truncated mutant plasmids of the enJSRV LTR based on the laboratory-preserved pGL4.10/enJSRV LTR template.The enJSRV LTR promoter core region was selected using a dual luciferase reporter gene system.Potential transcription factor binding sites in the enJSRV LTR promoter core region were predicted using bioinformatics software and a true nuclear expression plasmid was constructed.The effects of these plasmids on the activity of the enJSRV LTR promoter were then detected using a dual luciferase reporter gene system.Sequencing results showed that the pGL4.10/enJSRV LTR truncated mutant plasmids were successfully constructed.The fluorescence activity detection results showed that the enJSRV LTR promoter activity in the-118—-44 bp region was significantly reduced(P＜0.001),suggesting the existence of a positive regulation element of the LTR promoter.Flag/SOX17,Flag/EZH2,Flag/FOXMl and Flag/ZNF143 true nuclear expression plasmids were successfully constructed,of which overexpression of SOX17 significantly increased the activity of the enJSRV LTR promoter(P＜0.001).This study finally identified the core promoter region of enJSRV LTR and determined that transcription factor SOX17 can directly promote enJSRV LTR transcription.These findings provide a basis for the molecular mechanism of enJSRV transcriptional regulation and promote further research in the JSRV field.关键词
内源性绵羊肺腺瘤病毒/长末端重复序列/双荧光素酶报告基因系统/SOX17Key words
enogenous jaagsiekte sheep retrovirus(enJSRV)/long terminal repeat(LTR)/dual-lu-ciferase reporter assay system/SOX17分类
农业科技引用本文复制引用
马鑫淇,杜晓悦,段续接,张培,陈思旭,刘淑英..内源性绵羊肺腺瘤病毒LTR转录调控元件的筛选[J].中国兽医科学,2025,55(6):813-819,7.基金项目
国家自然科学基金项目(32072819,32360863) （32072819,32360863）
内蒙古科技重大专项计划资助项目(2021ZD0010) （2021ZD0010）
内蒙古草原英才创新团队项目(20151031) （20151031）
牛羊疫病防控与发育工程创新团队资助项目(BR22-13-08) （BR22-13-08）
兽医基础与牛羊疫病防控技术研发创新团队项目(NMGIRT2412) （NMGIRT2412）
研究生科研创新资助项目(B20231072Z) （B20231072Z）
内蒙古自治区教育厅一流学科科研专项(YLXKZX-NND-012) （YLXKZX-NND-012）
