中国兽医科学2025，Vol.55Issue(6)：820-829,10.DOI:10.16656/j.issn.1673-4696.2025.0094
醌氧化还原辅酶1通过调控糖酵解促进PRRSV增殖的机制研究
Mechanistic study of NAD(P)H quinone oxidoreductase 1 promoting PRRSV proliferation through regulation of glycolysis
摘要
Abstract
This study aimed to investigate the role of the host protein NAD(P)H quinone oxidoreduc-tase 1(NQO1)in the proliferation of porcine reproductive and respiratory syndrome virus(PRRSV)and its under-lying.Initially,by constructing NQO1-HA and NQO1P187S-HA plasmids and transfecting them into Marc-145 cells,we found that overexpression of NQO1 significantly enhanced the expression of PRRSV ORF7 mRNA and the PRRSV N protein,resulting in a marked increase in viral titer.Conversely,NQO1P187S-HA,a mutant with inac-tivated enzymatic activityfailed to enhance PRRSV proliferation,indicating that NQO1's enzymatic ac-tivity plays a crucial role in regulating PRRSV proliferation.Additionally,RNA-seq analysis revealed that NQO1 potentially regulates glycolysis through the PI3K-Akt signaling pathway.Further analysis showed that NQO1 overexpression increased the transcription levels of key glycolytic enzymes by qRT-PCR experiments.Furthermore,glucose consumption and lactate dehydrogenase(LDH)assays confirmed that NQO1 significantly enhances cellular glycolytic activity.However,treatmented with the 2-DG,a glycolysis inhibitor significantly suppressed the promotive effect of NQO1 on PRRSV proliferation,in-dicating that glycolysis plays an important role in NQO1-mediated regulation of PRRSV proliferation.In conclusion,this study demonstrates that NQO1 promotes PRRSV proliferation by regulating glycoly-sis,providing a theoretical basis for NQO1 as a potential antiviral target.关键词
猪繁殖与呼吸综合征病毒/醌氧化还原辅酶1/糖酵解Key words
porcine reproductive and respiratory syndrome virus(PRRSV)/NQO1/glycolysis分类
农业科技引用本文复制引用
赵微,王君君,卢天天,李焕荣,董虹,周双海,刘雪威..醌氧化还原辅酶1通过调控糖酵解促进PRRSV增殖的机制研究[J].中国兽医科学,2025,55(6):820-829,10.基金项目
国家自然科学青年科学基金项目(32102663) （32102663）
北京科学技术协会青年人才托举工程项目(QNTJ-2023005) （QNTJ-2023005）
北京市教育委员会科学研究计划项目(KM202210020005) （KM202210020005）
