中国临床医学2025，Vol.32Issue(3)：421-431,11.DOI:10.12025/j.issn.1008-6358.2025.20241417
LINC00638下调抑制Nrf2/ARE通路参与类风湿性关节炎相关间质性肺病发展
Downregulation of LINC00638 contributes to the pathogenesis of rheumatoid arthritis-associated interstitial lung disease via inhibiting the Nrf2/ARE signaling pathway
摘要
Abstract
Objective To identify long non-coding RNA(lncRNA)associated with rheumatoid arthritis-associated interstitial lung disease(RA-ILD)and investigate their mechanisms.Methods Peripheral blood samples were collected from RA-ILD patients(n=3),RA patients without lung involvement(n=3),and healthy controls(n=3).Next-generation sequencing was performed to screen differentially expressed lncRNA.A human fibrotic lung cell model was established by inducing the MRC-5 cell line with transforming growth factor-β(TGF-β).Following siRNA-mediated knockdown of target genes,changes in inflammatory and oxidative stress-related genes were analyzed via real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR).Western blotting and dual-luciferase reporter(DLR)assays were used to validate protein expression,ubiquitination levels,and nuclear translocation of oxidative stress regulators,and antioxidant response element(ARE)transcriptional activity.Rescue experiments were conducted to confirm the role of target lncRNA in oxidative stress and inflammation in fibrotic lung cells.Results High-throughput sequencing revealed significant downregulation of LINC00638 in RA-ILD patients.Knockdown of LINC00638 markedly reduced transcriptional levels of interleukin(IL)-4,nuclear factor erythroid-2-related factor 2(Nrf2),heme oxygenase-1(HO-1),and superoxide dismutase 2(SOD2),while increasing IL-6,IL-1β,interferon-γ(IFN-γ),and reactive oxygen species(ROS)levels.Furthermore,LINC00638 knockdown decreased Nrf2 protein expression,increased its ubiquitination,reduced nuclear translocation,and suppressed ARE transcriptional activity.In MRC-5 cells,LINC00638 knockdown combined with N-acetylcysteine treatment restored Nrf2 and HO-1 levels while reducing IL-6 expression.Conclusions LINC00638 suppresses inflammatory responses in RA-ILD by activating the Nrf2/ARE antioxidant signaling pathway,suggesting its potential as a therapeutic target for diagnosis and treatment.关键词
LINC00638/类风湿性关节炎/间质性肺病/氧化应激Key words
LINC00638/rheumatoid arthritis/interstitial lung disease/oxidative stress分类
医药卫生引用本文复制引用
廖卓君,汤乃望,陈佳惠,孙学英,陆佳敏,吴琴,余荣环,周颖..LINC00638下调抑制Nrf2/ARE通路参与类风湿性关节炎相关间质性肺病发展[J].中国临床医学,2025,32(3):421-431,11.基金项目
上海市徐汇区中心医院基金项目(2022-04).Supported by Fund of Shanghai Xuhui Central Hospital(2022-04). （2022-04）
