中国林副特产Issue(3)：8-12,5.DOI:10.13268/j.cnki.fbsic.2025.03.003
球孢白僵菌DU01对杨扇舟蛾幼虫的致病力及胞外酶活性分析
Analysis of Pathogenicity and Extracellular Enzyme Activity of Beauveria bassiana DU01 on Larva of Clostera anachoreta
摘要
Abstract
In order to obtain the entomogenic fungi with high pathogenicity to the larva of Clostera anachoreta in the high cold area,the entomogenic fungi were isolated from the cadavers of adult spruce-flowered longhorn beetle in the Weihe Town Forest Farm of Harbin City in Heilongjiang Province.The entomopathogenic fungi were identified through morphological observation and molecular biological analysis,and then their virulence to the larva of the tox-icity test and extracellular enzyme activity were determined.The results showed that the isolated and purified strain DU01 was Beauveria bassiana.Under the infection of strain DU01 with different concentration of spore suspension(1×108,1×107,1×106,1×105,1×104 spores/mL),the mortality of the larva was increased with the increase of the concentration of spore suspension.When the concentration was 1.0×108 spores/mL,the time to death of larva was 3.84 d,and the adjusted mortality of larva was 100%after 7 days of inoculation.Protease activity and chitinase activity reached the maximum value on the 4th day,5.65 U/mL and 4.77 U/mL,respectively.The results indicated that Beauveria bassiana had strong pathogenicity to the larva of Clostera anachoreta,which could provide a new idea for the green control of Clostera anachoreta.关键词
杨扇舟蛾/球孢白僵菌/分离鉴定/毒力/胞外酶活性Key words
Clostera anachoreta/Beauveria bassiana/Isolation and identification/Toxicity/Extracellular enzyme activity分类
林学引用本文复制引用
刘欣,张瑶,李亚洲,邓勋,周琦..球孢白僵菌DU01对杨扇舟蛾幼虫的致病力及胞外酶活性分析[J].中国林副特产,2025,(3):8-12,5.基金项目
黑龙江省省属科研院所科研业务费项目(LKSB2023-6)、黑龙江省重点研发计划项目(2023ZX02B05)、黑龙江省科研院所科研业务费项目(CZKYF2021-2-B018)、黑龙江省"森林保护学"省级领军人才梯队后备带头人资助项目黑人社函(2023)547号. （LKSB2023-6）
