中国农业科学2025，Vol.58Issue(11)：2162-2175,14.DOI:10.3864/j.issn.0578-1752.2025.11.007
链霉菌TOR3209及其挥发性有机化合物提高烟草抗木贼镰孢的能力
Streptomyces TOR3209 and Its Volatile Organic Compounds Enhance Tobacco Resistance to Fusarium equiseti
摘要
Abstract
[Objective]The objective of this study is to explore the effect of Streptomyces TOR3209 and its volatile organic compounds(VOCs)on tobacco disease resistance,clarify the direct and indirect mechanism of the strain and its VOCs to improve tobacco disease resistance,and to provide a theoretical basis for the biocontrol application of TOR3209 and its VOCs.[Method]Streptomyces TOR3209 and Fusarium equiseti were selected as research materials,and the inhibition ability of TOR3209 and its VOCs against F.equiseti was evaluated by using plate confrontation method and growth rate method.Tobacco was inoculated with different concentrations of TOR3209 suspension(1.0×101,1.0×103,1.0×105,1.0×107 CFU/mL)and co-cultured with VOCs produced by TOR3209.The effects of the strain and its VOCs on tobacco resistance to F.equiseti were tested.The transcriptome sequencing of tobacco was performed to analyze the differential gene expression.Solid phase microextraction(SPME)method was used to identify the VOCs produced by TOR3209,and a compound with strong specificity(heptadecane)was selected to verify the resistance of tobacco to F.equisetaria.Different concentrations of heptadecane(1 mol·kg-1,1 mmol·kg-1,1 μmol·kg-1,0.001 μmol·kg-1)were added as treatment groups to verify the effect of heptadecane on tobacco.[Result]Streptomyces TOR3209 and its VOCs had antagonistic effects on F.equiseti,with inhibition rates of 56.9%and 60.0%,respectively.Compared with the control,the severity of tobacco disease was significantly reduced under different concentrations of TOR3209 suspension(P＜0.01),and the treatment with the concentration of 1.0×101 CFU/mL had the best disease resistance effect.Both the retained strain treatment group and the removed strain treatment group could improve the disease resistance of tobacco,the control effect was 85.7%and 72.5%,respectively.The main way was to induce tobacco systemic resistance during the action of TOR3209 VOCs.Transcriptome analysis of tobacco showed that the VOCs of TOR3209 induced up-regulation of several genes in tobacco,and many of the genes were related to the improvement of plant disease resistance.Among the VOCs produced by TOR3209,several compounds were identified,among which heptadecane could improve the resistance of tobacco to F.equiseti infection after co-culture with tobacco,and the treatment group with a mass concentration of 1 mmol·kg-1 had a significant effect.[Conclusion]Streptomyces TOR3209 and its VOCs mainly improve the resistance of tobacco by inducing systemic resistance,and the VOCs of TOR3209 can regulate the gene expression of tobacco.关键词
链霉菌TOR3209/烟草/木贼镰孢/抗病/拮抗/生物防治Key words
Streptomyces TOR3209/tobacco/Fusarium equiseti/disease resistance/antagonism/biological control引用本文复制引用
赵琳琳,贺羽茜,彭杰丽,王旭,马佳,张秀敏,胡栋..链霉菌TOR3209及其挥发性有机化合物提高烟草抗木贼镰孢的能力[J].中国农业科学,2025,58(11):2162-2175,14.基金项目
国家重点研发计划(2021YFD1901004-4)、河北省农林科学院科技创新专项(2022KJCXZX-ZHS-3)、河北省湿地保护与绿色发展协同创新中心开放基金(2023hbxczx2-7) （2021YFD1901004-4）
