中国兽医杂志2025，Vol.61Issue(6)：47-52,6.DOI:10.20157/j.cnki.zgsyzz.2025.06.006
青海省部分地区牦牛感染中华泰勒虫分子流行病学调查
Molecular Epidemiological Investigation of Theileria sinensis Infection from Yak in Some Areas of Qinghai Province
摘要
Abstract
To assess the prevalence of Theileria sinensis(T.sinensis)infection in yaks in parts of Qinghai Province,this study collected 411 blood samples from yaks across 10 counties in 6 prefectures.Genomic DNA was extracted from the blood samples,and nested PCR was used to amplify the V4 hypervariable region of the Piroplasm 18S rRNA gene for sequencing and analysis.The software DnaSP v6 was used to analyze the number of haplotypes,mutation sites,haplotype diversity,and nucleotide diversity of the positive samples.PopArt and MEGA 6.0 software were used to construct a haplotype network and phylogenetic tree,respectively.The results showed a T.sinensis infection rate of 19.46%(80/411)among yak blood samples.A total of 16 haplotypes of the T.sinensis 18S rRNA gene were identified,with Theileria sinensis haplotype 1(TsH1)being dominant(65/80),found across Haiyan,Henan,Dari,Guinan counties,and Yushu City.The remaining 15 haplotypes each accounted for 1.25%(1/80)and were exclusively found in Guinan County.The haplotype diversity index was 0.342,and nucleotide diversity was 0.002 53,with a total of 25 mutation sites.Phylogenetic analysis revealed that the 16 haplotypes clustered with previously published T.sinensis strains from Gansu,Wuhan,Hainan,Xinjiang,and Henan in GenBank.This study provides the essential data for the prevention and control of tick-borne T.sinensis infection in Qinghai Province.关键词
牦牛/中华泰勒虫/18S rRNA/感染率/单倍型/系统发育分析Key words
yak/Theileria sinensis/18S rRNA/infection rate/haplotype/phylogenetic analysis分类
畜牧业引用本文复制引用
韩元,李春花,李志,林伟山,马豆豆,蔡进忠,雷萌桐..青海省部分地区牦牛感染中华泰勒虫分子流行病学调查[J].中国兽医杂志,2025,61(6):47-52,6.基金项目
国家重点研发计划项目(2022YFD1602312) （2022YFD1602312）
青海省科技厅项目(2022-NK-118) （2022-NK-118）
大通县科技计划项目(2024-ZB-1) （2024-ZB-1）
