中国兽医杂志2025，Vol.61Issue(6)：91-100,10.DOI:10.20157/j.cnki.zgsyzz.2025.06.013
二氢杨梅素缓解ETEC K88诱导的肠道炎性损伤
Dihydromyricetin Alleviates ETEC K88-Induced Intestinal Inflammatory Injury
摘要
Abstract
To investigate the effect of dihydromyricetin(DHM)on intestinal inflammatory injury induced by enterotoxigenic Escherichia coli K88(ETEC K88),an in vitro inflammation model was established using porcine small intestinal epithelial cells(IPEC-1).Western blotting was used to detect protein expression levels of cystic fibrosis transmembrane conductance regulator(CFTR),sodium-hydrogen exchanger 3(NHE3),nuclear factor-κB p65(NF-κB p65),phosphorylated NF-κB p65(p-NF-κB p65),interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-18(IL-18),and tumor necrosis factor-α(TNF-α).An in vivo model of intestinal inflammation was also constructed by orally administering DHM at doses of 25,50,and 100 mg/(kg·bw)to mice,followed by infection with ETEC K88.Blood samples were collected 24 hours post-infection to assess serum biochemical parameters and blood cell classification.The small intestines of mice were dissected to observe villus morphology.The results showed that the in vitro model was successfully established.Compared with the ETEC K88-infected model group,DHM at concentrations of 50,100,200,and 400 µg/mL extremely significant downregulated the protein expression levels of NF-κB p65,IL-1β,and IL-6(P<0.01),and extremely significant upregulated NHE3(P<0.01).Additionally,DHM at 100,200,and 400 µg/mL extremely significant reduced CFTR and TNF-α expression(P<0.01),while 400 µg/mL DHM also extremely significant lowered IL-18 levels(P<0.01).In vivo,the mouse inflammation model was also successfully established.Compared with the ETEC K88 group,mice administered with 100 mg/(kg·bw)DHM showed significantly reduced serum levels of alanine aminotransferase(ALT),total cholesterol(TC),creatinine(CREA),blood urea nitrogen(BUN),aspartate aminotransferase(AST),and triglycerides(TG);reduced counts of white blood cells(WBC),neutrophils,eosinophils,and intestinal crypt depth(CD)(P<0.05).Meanwhile,DHM significantly increased serum levels of total bilirubin(T-Bil),glucose(GLU),alkaline phosphatase(ALP),lymphocyte counts,villus height(VH),and VH/CD ratio(P<0.05).Histopathology revealed that mice in the DHM group had intact intestinal villi,orderly crypt-villus structures,and normal mucosal epithelial cells.In conclusion,DHM significantly alleviated ETEC K88-induced intestinal inflammatory injury in mice and exerted a protective effect on intestinal health.This study provides preliminary insight into the mechanism of DHM action and offers scientific evidence supporting its potential application in treating ETEC infections.关键词
产肠毒素大肠杆菌/二氢杨梅素/囊性纤维化跨膜转导调节因子/NF-κB通路Key words
enterotoxigenic Escherichia coil/dihydromyricetin/cystic fibrosis transmembrane conductance regulator/NF-κB pathway分类
畜牧业引用本文复制引用
师亚倩,郭璞,周华林,刘锦,叶纯,项智锋,吴仲元,邱银生..二氢杨梅素缓解ETEC K88诱导的肠道炎性损伤[J].中国兽医杂志,2025,61(6):91-100,10.基金项目
湖北省重点研发计划(2023BBB069) （2023BBB069）
湖北省教育厅科研计划(D20221607) （D20221607）
湖北省自然科学基金(JCZRYB202401220) （JCZRYB202401220）
湖北省科技服务人才专项(2023DJC107) （2023DJC107）
