中国动物检疫2025，Vol.42Issue(6)：87-91,5.DOI:10.3969/j.issn.1005-944X.2025.06.014
O型口蹄疫病毒磁微粒化学发光抗体检测方法的建立
Establishment of a Magnetic Particle Chemiluminescent Immunoassay for Detection of Antibodies against FMDV-O
耿玉静 1白晶晶 1刘慧芳 1李长印 2张鹏翼 3原小燕 1吴彬 1李玉婉4
作者信息
- 1. 洛阳莱普生信息科技有限公司,河南 洛阳 471000
- 2. 郸城县农业农村局,河南郸城 477150
- 3. 洛阳现代生物技术研究院有限公司,河南 洛阳 471000
- 4. 河南科技大学,河南 洛阳 471000
- 折叠
摘要
Abstract
In order to establish a high-throughput and fully automated magnetic particle chemiluminescent immunoassay(MP-CLIA)for detecting antibodies against foot-and-mouth disease virus serotype O(FMDV-O),a magnetic particle chemiluminescence method for FMDV-O was established through optimizing all reaction conditions using FMDV-O-VP1 structural protein and enzyme-labelled monoclonal antibody.The results showed that the antibodies against FMDV-O were detected positive and negative when the critical values(S/N)were≤0.8 and＞0.8,respectively.The established method was with good specificity without cross-reaction with immune sera of five common pathogens;and its limit of detection(LOD)for sensitivity control serum was 1:1 024,and intra-and inter-assay coefficients of variation(CVs)were within 10%,which was 95.9%compliant with the standard method.In conclusion,the established method presented high sensitivity,specificity and reproducibility,and was an efficient method for detecting immune antibodies against FMDV-O.关键词
O型FMDV/磁微粒化学发光/酶标抗体/敏感性/特异性Key words
FMDV-O/magnetic particle chemiluminescence/enzyme-labeled antibody/antibody detection分类
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耿玉静,白晶晶,刘慧芳,李长印,张鹏翼,原小燕,吴彬,李玉婉..O型口蹄疫病毒磁微粒化学发光抗体检测方法的建立[J].中国动物检疫,2025,42(6):87-91,5.
