中国动物检疫2025，Vol.42Issue(6)：106-112,7.DOI:10.3969/j.issn.1005-944X.2025.06.017
化脓隐秘杆菌灭活疫苗佐剂的筛选与免疫保护性评价
Screening of Adjuvants for Inactivated Vaccine of Arcanobacterium pyogenes and Evaluation on Their Immunoprotective Efficacy
摘要
Abstract
In order to select an adjuvant appropriate for inactivated vaccine of Arcanobacterium pyogenes,4 kinds of inactivated vaccines(including ISA 15 adjuvant vaccine,ISA 61 adjuvant vaccine,ISA 201 adjuvant vaccine and aluminum hydroxide adjuvant vaccine)were prepared using 4 adjuvants,respectively,which were observed for physical properties and tested for safety,and then immunized into mice to detect serum antibodies and the immunoprotective efficacy.The results showed that all the 4 adjuvant vaccines were qualified in physical properties,but caused adverse reaction(dorsal inflammation)after injection,which was the slightest in mice immunized with ISA 61 adjuvant vaccine;serum antibody level of mice in ISA 61 group was extremely significantly higher than that of other groups(P＜0.01),followed by mice in ISA 201 group;7 days after the third immunization,mice were challenged with Arcanobacterium pyogenes,the protection rate provided by ISA 61 adjuvant vaccine was the highest,reaching 90%,followed by ISA 201,ISA 15,aluminum hydroxide adjuvant vaccine and adjuvant-free inactivated vaccine,remaining 60%,50%,40%and 30%,successively.In conclusion,ISA 61 adjuvant vaccine could cause less side effects,induce antibody level at the highest level,and provide the highest protective efficacy,which was preferred for preparing inactivated vaccine of Arcanobacterium pyogenes.A foundation was laid by the study for further development of inactivated vaccine of Arcanobacterium pyogenes.关键词
化脓隐秘杆菌/佐剂/疫苗/免疫保护Key words
Arcanobacterium pyogenes/adjuvant/vaccine/immunoprotection分类
农业科技引用本文复制引用
熊静,张素辉,徐登峰,沈克飞,付利芝,余远迪..化脓隐秘杆菌灭活疫苗佐剂的筛选与免疫保护性评价[J].中国动物检疫,2025,42(6):106-112,7.基金项目
重庆市科研机构绩效激励引导专项(CSTB2023JXJL-YFX0063) （CSTB2023JXJL-YFX0063）
重庆市财政资金专项(22537C) （22537C）
四川省区域创新合作项目(2024YFHZ0110) （2024YFHZ0110）
