化脓隐秘杆菌灭活疫苗佐剂的筛选与免疫保护性评价OA

In order to select an adjuvant appropriate for inactivated vaccine of Arcanobacterium pyogenes,4 kinds of inactivated vaccines(including ISA 15 adjuvant vaccine,ISA 61 adjuvant vaccine,ISA 201 adjuvant vaccine and aluminum hydroxide adjuvant vaccine)were prepared using 4 adjuvants,respectively,which were observed for physical properties and tested for safety,and then immunized into mice to detect serum antibodies and the immunoprotective efficacy.The results showed that all the 4 adjuvant vaccines were qualified in physical properties,but caused adverse reaction(dorsal inflammation)after injection,which was the slightest in mice immunized with ISA 61 adjuvant vaccine;serum antibody level of mice in ISA 61 group was extremely significantly higher than that of other groups(P＜0.01),followed by mice in ISA 201 group;7 days after the third immunization,mice were challenged with Arcanobacterium pyogenes,the protection rate provided by ISA 61 adjuvant vaccine was the highest,reaching 90%,followed by ISA 201,ISA 15,aluminum hydroxide adjuvant vaccine and adjuvant-free inactivated vaccine,remaining 60%,50%,40%and 30%,successively.In conclusion,ISA 61 adjuvant vaccine could cause less side effects,induce antibody level at the highest level,and provide the highest protective efficacy,which was preferred for preparing inactivated vaccine of Arcanobacterium pyogenes.A foundation was laid by the study for further development of inactivated vaccine of Arcanobacterium pyogenes.