羊口疮病毒云南流行毒株毒力基因遗传多样性分析OA

In order to further explore molecular and genetic variation characteristics of major virulence factors of Orf virus(ORFV)Yunnan prevalent strains,molecular genetic analysis was carried out targeting at the virulence genes,including VIR,GIF,vIL-10,VEGF-E and CBP,based on four Yunnan strains isolated from 2021 to 2023.It was found that,by gene sequencing and data analysis,all the strains were obviously different in terms of genus/species classification,associated strains and sequence homology under different virulence gene sequences.The virulence genes of the strains presented complex mutational characteristics,indicating self-mutation and gene reassortment of the virus during its transmission and propagation,and thereby generating genetic diversity.In conclusion,the study would play an important role in understanding the transmission routes of the virus and analyzing its evolutionary characteristics,providing a key reference for subsequent pathogenicity researches and formulation of prevention and control strategies.