中国动物检疫2025，Vol.42Issue(6)：128-132,5.DOI:10.3969/j.issn.1005-944X.2025.06.020
首免日龄、免疫剂量和酸化剂对肠炎沙门菌弱毒疫苗免疫效果的影响
Effects of Age at First Immunization,Immunization Dose and Acidifier on the Efficacy of Salmonella enteritidis Vaccine
摘要
Abstract
In order to explore the optimal immunization conditions for the attenuated vaccine Sm24_Rif12_Ssq for Salmonella enteritidis,the age at first immunization,immunization dose and acidifier application interval were tested and analyzed.Hy-Line variety brown cocks(0-day-old)were selected and orally immunized with 1 avian-dose vaccine at 0 and 7 days of age.Cloacal swabs were collected 1 to 7 days after the immunization to detect Salmonella using qPCR,and to analyze the optimal age for the operation;cocks at 0-day-old were orally administered with 1.0 and 0.5 avian-dose vaccines,then their livers were collected 1 to 14 days after the immunizatio n for bacterial count,and cloacal swabs for Salmonella detection by qPCR to analyze the optimal dose;cocks at 0-day-old were orally administered with 1 avian-dose vaccine,and fed with feed containing acidifier at 0,3 and 7 days after the immunization,cloacal swabs were collected 1 to 15 days after the immunization to detect Salmonella by qPCR,and to analyze the optimal acidifier application interval.The results showed that the cloacal Salmonella positive rate of 0-day-old immunized cocks was from 30%to 85%,but that of 7-day-old immunized cocks was only from 0%to 20%;there was no obvious difference(P＞0.05)in the liver bacterial load and cloacal Salmonella positive rate of cocks immunized with 1.0 and 0.5 avian-dose vaccines;the positive rate was not affected(P＞0.05)by acidifier application at different intervals after the immunization.In conclusion,the vaccine for Salmonella enteritidis was suitable for the first immunization with 0.1 avian-dose vaccine at 0-day-old,and its intestinal colonization was not affected by acidifier application after the immunization.Data reference was provided for clinical administration of the attenuated vaccine Sm24_Rif12_Ssq for Salmonella enteritidis.关键词
肠炎沙门菌/Sm24_Rif12_Ssq/首免日龄/免疫剂量/酸化剂Key words
Salmonella enteritis/Sm24_Rif12_Ssq/age at first immunization/immunization dose/acidifier分类
畜牧业引用本文复制引用
闫雪敏,赵东旭,朱飞,苏士炫,蔡梦雷,孟晨晨,周守长,刘建钗,崔国林..首免日龄、免疫剂量和酸化剂对肠炎沙门菌弱毒疫苗免疫效果的影响[J].中国动物检疫,2025,42(6):128-132,5.基金项目
河北省创新能力提升计划项目(235A6601D) （235A6601D）
中央引导地方科技发展资金项目(216Z6602G) （216Z6602G）
国家现代农业产业技术体系项目(CARS-40) （CARS-40）
