中国兽医杂志2025，Vol.61Issue(6)：112-119,8.DOI:10.20157/j.cnki.zgsyzz.2025.06.015
羧甲基化黄芪多糖的制备及其体外抗氧化活性研究
Preparation and In Vitro Antioxidant Activity of Carboxymethylated Astragalus Polysaccharides
摘要
Abstract
To explore the preparation and in vitro antioxidant activity of carboxymethylated Astragalus polysaccharides(CM-APS),this study employed a solvent method to chemically modify Astragalus polysaccharides(APS).The degree of substitution(DS)of CM-APS was used as the evaluation index,and preparation conditions were optimized through single-factor and orthogonal experiments.The successful carboxymethylation of APS was confirmed by Fourier-transform infrared spectroscopy(FTIR).An oxidative damage model was established using hydrogen peroxide(H₂O₂)-induced Caco-2 human colorectal cancer cells to assess the antioxidant activity of APS and CM-APS in vitro.The results showed that the optimal preparation conditions for CM-APS were:9.5 g of chloroacetic acid,etherification temperature of 60℃,and 50 mL of 30%NaOH,under which the degree of substitution reached 1.716.FTIR analysis of CM-APS revealed characteristic peaks of both polysaccharides and carboxymethyl functional groups.Both APS and CM-APS at concentrations of 400-1 600 mg/L significantly improved H₂O₂-induced cytotoxicity in Caco-2 cells.At 200 mg/L,APS and CM-APS significantly upregulated the activities of total glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)(P<0.05 or P<0.01),and significantly reduced the relative contents of malondialdehyde(MDA)and reactive oxygen species(ROS)(P<0.01).Additionally,the overall effect of CM-APS was significantly stronger than that of APS.In conclusion,CM-APS with a degree of substitution of 1.716 was successfully synthesized.CM-APS showed stronger in vitro antioxidant activity than APS,indicating improved chemical structure and enhanced biological activity,which provides a theoretical basis for the development and utilization of APS in the veterinary medicine field.关键词
黄芪多糖/羧甲基化/制备/抗氧化/Caco-2细胞Key words
Astragalus polysaccharides/carboxymethylation/preparation/antioxidant/Caco-2 cells分类
农业科技引用本文复制引用
王鸿鑫,叶钰滢,刘栩沂,韩梓杰,邓桦,张楠,张德显,杨鸿..羧甲基化黄芪多糖的制备及其体外抗氧化活性研究[J].中国兽医杂志,2025,61(6):112-119,8.基金项目
广东省高校重点领域专项(2021ZDZX4015) （2021ZDZX4015）
