中国动物传染病学报2025，Vol.33Issue(2)：11-18,8.
猪流行性腹泻病毒感染仔猪空肠组织的转录组学分析
Transcriptome Analysis of Jejunal Tissues of Piglets Infected with Porcine Epidemic Diarrhea Virus
摘要
Abstract
Porcine epidemic diarrhea virus(PEDV)infection causes severe diarrhea in piglets,leading to intestinal mucosal damage and immunodeficiency.In this study,the jejunal tissues of PEDV-infected piglets were collected for transcriptome sequencing analysis using RNA-Seq technology to explore the changes in genomic transcription level and screen differentially expressed genes in the host immune response.RNA-seq results showed that 339 significantly differentially expressed genes(DEGs)were identified in PEDV-infected piglets compared with normal uninfected piglets,including 182 up-regulated genes and 157 down-regulated genes.GO molecular function results showed that the immune-related differentially expressed genes were mainly related to the immunoglobulin and receptor binding,the T cell proliferation,etc.KEGG pathway enrichment results showed that the differentially expressed genes were mainly involved in TNF signaling pathway,cytokine-cytokine receptor interaction,toll-like receptor signaling pathway,Jak-STAT signaling pathway and RIG-I-like receptor signaling pathway.Finally,the differentially expressed genes were verified by qRT-PCR,and the results were basically consistent with the transcriptome results.This study provided a scientific basis for further study on the mechanism of changes in host immune response caused by PEDV infection.关键词
猪流行性腹泻病毒/转录组分析/差异表达/RNA-Seq/先天免疫Key words
Porcine epidemic diarrhea virus/transcriptome analysis/differential expression/RNA-Seq/innate immune分类
农业科技引用本文复制引用
曾德平,黄伟坚,欧阳康,陆颖,余科辰,卢峥朴,柏家果,秦秋英,隆美金,陈樱,韦祖樟..猪流行性腹泻病毒感染仔猪空肠组织的转录组学分析[J].中国动物传染病学报,2025,33(2):11-18,8.基金项目
广西自然科学基金资助项目(2021GXNSFAA196067) （2021GXNSFAA196067）
大学生创新创业训练计划项目(202210593870) （202210593870）
