中国动物传染病学报2025，Vol.33Issue(2)：35-42,8.
欧亚类禽H1N1亚型猪流感全病毒灭活疫苗的免疫保护效力评价
Immune Efficacy Evaluation of Inactivated Vaccines Against Eurasian Avian-Like H1N1 Subtype Swine Influenza Virus
摘要
Abstract
In this study,Madin Darby canine kidney(MDCK)cells was used as the culture medium to amplify the Porcine influenza virus.Formaldehyde and β-Propiolactone(BPL)had been selected as chemical inactivators for virus inactivation.The optimal conditions for formaldehyde inactivation were 0.1％final concentration and 37℃ inactivation for 24 h.The optimal inactivation conditions for BPL were 0.02％ final concentration and 4℃ inactivation for 18 h.The antigen hemagglutination titer was about one titer lower than the original titer after inactivation.On the basis of using different inactivating agents,the inactivated vaccine was emulsified with oil phase adjuvant ISA 201 VG and water phase adjuvant HA 208.BALB/c mice were immunized with inactivated vaccines to evaluate immune and antiviral protective effects,with a challenge dose of 1 ×106 TCID50 per mice.Formaldehyde inactivated vaccines could stimulate mice to produce high levels of HI antibodies compared to BPL inactivated vaccines.Higher antibody levels were also reflected in the use of adjuvants,that the oil phase adjuvant ISA 201 VG is more effective than the water phase adjuvant HA 208.The survival rate of the vaccine immunized group reached 100％ after SIV challenged.By comparing the experimental data of the control group,the virus content in the lungs of immunized group was significantly reduced.The above results indicate that the inactivated vaccine with formaldehyde+ISA 201 VG adjuvant immunization of BALB/c mice could produce high level of antibodies and resist the attack of Eurasian avian-like SIV,providing a scientific basis for the research and production of inactivated SIV vaccines in the future.关键词
欧亚类禽H1N1/甲醛/BPL/全病毒灭活疫苗/免疫效力评价Key words
Eurasian avian-like H1N1/formaldehyde/β-Propiolactone/whole virus inactivated vaccine/evaluation of immune efficacy分类
农业科技引用本文复制引用
陈荣琳,童武,郑浩,刘长龙,姜一峰,孔宁,童光志,于海,邢燕茹,王娟,李健楠,高硕磊,刘清艳,祝宇翔,周艳君,单同领..欧亚类禽H1N1亚型猪流感全病毒灭活疫苗的免疫保护效力评价[J].中国动物传染病学报,2025,33(2):35-42,8.基金项目
国家重点研发计划项目课题(2022YFD1800803) （2022YFD1800803）
国家自然科学基金面上项目(31970175) （31970175）
中国农业科学院创新工程"猪病毒性繁殖障碍疫病防控技术团队"项目 （）
