中国动物传染病学报2025，Vol.33Issue(2)：89-96,8.
阿卡斑病毒抗体双抗原夹心化学发光酶免疫分析法的建立
Development of Double Antigen Sandwich Chemiluminscence Enzyme Immunoassay for Detecting the Antibody of Akabane Virus
摘要
Abstract
Akabane virus(AKAV)is an arbovirus-borne virus,which mainly infetcts ruminants.In recent years,the prevalence of AKAV has become complex in China.To establish a more sensitive detection method for AKAV antibody in a variety of animals,recombination AKAV N protein and HRP labelled recombination AKAV N protein were uesd as the capture antigen and detection antigen,respectively.After the reaction conditions were optimzied,an AKAV antibody double antigen sandwich chemiluminescence enzyme immunoassay detection method was firstly established.The method had no cross-reactivity with the positive serum of BTV,EHDV,BEV and PRV with the intra-and inter-batch coefficients of variation all less than 8％.Compared with the AKAV blocking ELISA kit,the sensibility of the method was increased by 32 folds,with the overall detection time shortened by 42 min,and the κ value of 0.826.Meanwhile,it was proved to be precise by neutralization test.The AKAV antibody chemiluminescence enzyme immunoassay antibody detection method established in this study has strong specificity,high sensitivity,good reproducibility,simplicity,rapidity and precise.This method could be an effective monitoring tool of large-scale serum samples,serosurvey and the prevention of AKAV.关键词
阿卡斑病毒/N蛋白/双抗原夹心CLEIAKey words
Akabane virus/N protein/double antigen sandwich CLEIA分类
农业科技引用本文复制引用
陈赫威,陈樱,马玲,杨清,曹佳媛,韦珊珊,陆晨阳,林俊,覃绍敏,陈凤莲,吴健敏..阿卡斑病毒抗体双抗原夹心化学发光酶免疫分析法的建立[J].中国动物传染病学报,2025,33(2):89-96,8.基金项目
国家自然科学基金资助项目(32160830) （32160830）
广西壮族自治区兽医研究所基本科研业务费专项资助项目(桂科专项24-4) （桂科专项24-4）
广西兽医生物技术重点实验室开放基金资助项目(20-065-23-B-2) （20-065-23-B-2）
