中国动物传染病学报2025，Vol.33Issue(2)：97-105,9.
猪冠状病毒四重荧光定量PCR检测方法建立
Development of a Quadruple Real-time PCR Assay for Detection of Porcine Coronaviruses
摘要
Abstract
To differentially diagnose Porcine important coronavirus infection,this study designed specific primers and TaqMan probes to develop a quadruple fluorescent quantitative RT-PCR(qRT-PCR)assay for Swine acute diarrhea syndrome coronavirus(SADS-CoV),Porcine hemagglutinating encephalomyelitis virus(PHEV),Porcine delta-coronavirus(PDCoV)and Porcine epidemic diarrhea virus(PEDV).The assay was then evaluated for its specificity,sensitivity and stability.The results showed that the method had good specificity and amplified the tested Porcine coronaviruses with the minimum detection limits at 10 copies/μL for SADS-CoV,PDCoV and PHEV and 100 copies/μL for PEDV virus indicating its sensitivity 100 times higher than that of the conventional PCR.Both the within-group and between-group coefficients of variation were less than 2％,indicating good repeatability.This assay provided a good molecular detection tool for the differential diagnosis of porcine coronavirus infection and had important clinical application.关键词
猪急性腹泻综合征冠状病毒/猪血凝性脑脊髓炎病毒/猪δ-冠状病毒/猪流行性腹泻病毒/荧光定量RT-PCRKey words
Swine acute diarrhea syndrome coronavirus/Porcine hemagglutinating encephalomyelitis virus/Porcine δ coronavirus/Porcine epidemic diarrhea virus/fluorescence quantitative RT-PCR assay分类
农业科技引用本文复制引用
俞赵荣,孙彤,金雪明,孙竹筠,苏苌,刘静宜,刘英楠,陈鸿军..猪冠状病毒四重荧光定量PCR检测方法建立[J].中国动物传染病学报,2025,33(2):97-105,9.基金项目
上海市2020年度"科技创新行动计划"动物研究领域基金项目(20140900300) （20140900300）
