中国动物传染病学报2025，Vol.33Issue(2)：117-123,7.
动物源大肠杆菌MALDI-TOFMS质谱库的建立及在北京地区区域性差异应用分析
Characterization of Data Group for Escherichia coli from Animals with Regional Differences in Beijing Based on MALDI-TOF MS Technology
摘要
Abstract
To investigate the characteristics of Escherichia coli with regional difference,11 E.coli samples were collected from animals in Huairou district and other 22 E.coli samples were collected from animals in Yanqing district for bacterial characterization in the present study.Biochemical reactions,16S rRNA sequencing and MALDI-TOF MS technique were used to identify these E.coli samples.Afterwards,the accuracy of these assays was compared and the peptide mass fingerprint spectrum data were analyzed for these samples.The results showed that the accuracy of three assays was above 95％and the MALDI-TOF MS identification reached more than 99％when multiple sites were tested for one sample.MALDI-TOF MS identification showed good repeatability,high accuracy and fast results especially when the number of test repeats was increased for one sample.Although the E.coli samples from two regions had similar peptide mass fingerprint spectrum characteristics,they were distinguished by clustering and principal component analysis,indicating that the peptide mass spectrums of E.coli samples collected from Huairou and Yanqing had regional differences.This study provided a technical support for traceability analysis of E.coliby MALDI-TOF MS.关键词
大肠杆菌/MALDI-TOF MS/肽质量指纹谱/北京Key words
Escherichia coli/MALDI-TOF MS/peptide mass fingerprint spectrum/Beijing region分类
农业科技引用本文复制引用
张启龙,董志军,朱家强,周德刚,钱云开,刘利勤,傅彩霞,贾宏丽,刘海莹,栗云鹏,杨逸舟,田辉..动物源大肠杆菌MALDI-TOFMS质谱库的建立及在北京地区区域性差异应用分析[J].中国动物传染病学报,2025,33(2):117-123,7.基金项目
北京市农业农村局科技项目(20190117) （20190117）
海关总署科研项目(2020HK229) （2020HK229）
