中国动物传染病学报2025，Vol.33Issue(2)：146-155,10.
神经酰胺代谢酶SGMS2对NDV复制和增殖的影响
Effect of the Ceramide Metabolizing Enzyme SGMS2 on NDV Replication and Proliferation
摘要
Abstract
Sphingomyelin(SM)and ceramide(Cer)are the main phospholipid components of the Newcastle disease virus(NDV)capsule membrane and sphingomyelin synthase 2(SGMS2)is the regulatory enzyme for SM synthesis.A previous study found significant upregulation of the chicken SGMS2 gene in NDV-infected DF-1,suggesting that the change was associated with NDV infection.In this study,the chicken SGMS2 gene was obtained by cloning and bioinformatic analysis revealed 93.0％ and 92.4％ homology with human and mouse,respectively.The transmembrane region was highly conserved up to 96.8％ across species.The levels of chicken SGMS2 ontology were detected by qPCR in cells and tissues and found to be constant.qPCR showed NDV infection caused dramatic changes in chicken SGMS2 expression levels with significant down-regulation in tissues with high viral content.DF-1 cells were transfected with pCMV6-FLAG-ch-SGMS2 plasmid and significant decreases in mRNA and protein levels of viral NP were found in qPCR and Western blot,indicating that overexpression of SGMS2 inhibited the proliferative effect of NDV.This study laid a solid foundation for revealing the function of chicken SGMS2 protein and also provided a basis for elucidating the role of chicken SGMS2 on NDV proliferation.关键词
鸡鞘磷脂合成酶2/基因克隆与表达/氨基酸序列分析/新城疫病毒Key words
Chicken SGMS2/gene cloning and expression/amino acid sequence analysis/Newcastle disease virus分类
农业科技引用本文复制引用
冯亦亦,代军,谭磊,孙英杰,宋翠萍,廖瑛,丁铲,仇旭升,刘秀梵..神经酰胺代谢酶SGMS2对NDV复制和增殖的影响[J].中国动物传染病学报,2025,33(2):146-155,10.基金项目
上海市自然科学基金(21ZR1476800) （21ZR1476800）
上海市科技兴农项目(2022-02-08-00-12-F01154) （2022-02-08-00-12-F01154）
国家自然科学基金(32272978) （32272978）
