中国动物传染病学报2025，Vol.33Issue(2)：156-164,9.
天津地区猪繁殖与呼吸综合征病毒流行毒株ORF5基因的克隆及遗传变异分析
Cloning and Genetic Variation of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Epidemic Strains in Tianjin
摘要
Abstract
In order to understand the prevalence and genetic variation of porcine reproductive and respiratory syndrome virus(PRRSV)in Tianjin,209 tissues were collected from 2021 to 2022 from clinically suspected diseased pigs for detection of PRRSV and ORF5 genetic variation.The results showed that the PRRSV positive rate was 37.3％(78/209).Then,15 representative positive samples from different regions were selected for ORF5 gene cloning and sequencing.Sequence and phylogenetic analysis showed that the 15 PRRSV strains belonged to the American type.The ORF5 genes of 8 PRRSV strains had high homology with the HP-PRRSV-like strains represented by HuN4,and the identities of their nucleotide sequences and deduced amino acid sequences were 89.4％-97.3％and 80.6％-95.5％,respectively while their identities with NADC30,VR2332 and NADC34 were 77.1％-87.1％and 77.1％-86.6％,respectively.The ORF5 gene of 6 PRRSV strains had the highest homology with NADC30-like strains represented by NADC30.The homology of nucleotide sequence and deduced amino acid sequence was 91.9％-92.7％ and 91.5％-93.0％,respectively while the homology with HuN4,VR2332,NADC34 and other strains was low at 83.6％-87.8％ and 79.6％-86.6％,respectively.The ORF5 gene of one PRRSV strain had the highest homology with NADC34-like strains represented by NADC34.The homology of nucleotide sequence and deduced amino acid sequence of this strain was 96.4％ and 95.5％,respectively,while the homology with NADC30,HuN4,VR2332 and other strains was 86.7％-89.9％and 86.6％-92.0％,respectively.The results showed that HP-PRRSV-like and NADC30-like PRRSV were the main epidemic strains from 2021 to 2022 in Tianjin while NADC34-like strains were prevalent,indicating obvious genetic diversity.These results provided a theoretical basis for the prevention and control of PRRS in Tianjin.关键词
猪繁殖与呼吸障碍综合征病毒/ORF5基因/序列分析/遗传变异/同源性分析Key words
Porcine reproductive and respiratory syndrome virus/ORF5 gene/sequence analysis/genetic variation/homology analysis分类
农业科技引用本文复制引用
李文忠,荣新利,刘野,吴庆东,张乐,孙英峰,于海..天津地区猪繁殖与呼吸综合征病毒流行毒株ORF5基因的克隆及遗传变异分析[J].中国动物传染病学报,2025,33(2):156-164,9.基金项目
国家重点研发计划课题(2022YFD1800803) （2022YFD1800803）
天津市科技支撑计划重点项目(22YFZCSN00100) （22YFZCSN00100）
天津市优秀企业科技特派员项目(22YDTPJC00420) （22YDTPJC00420）
天津市优秀农业科技特派员项目(22ZYCGSN00570) （22ZYCGSN00570）
