中国兽医科学2025，Vol.55Issue(6)：719-724,6.DOI:10.16656/j.issn.1673-4696.2025.0088
O型口蹄疫病毒3D突变毒株的构建及其生物学特性分析
Construction and biological characteristic analysis of 3D mutant strain of O-type foot-and-mouth disease virus
摘要
Abstract
In this study,the 3D protein mutant strain rFMDV-M403A of the O-type foot-and-mouth dis-ease virus(FMDV)was constructed using reverse genetic technology and its biological characteristics were analyzed.The results indicate that the amino acids in the recombinant virus can stably mutate during passage.The virus titer shows that the growth rate of mutant strain is reduced compared to the wild-type strain,and the virulence of recombinant virus is significantly lower than that of wild-type strain.In animal experiments,suckling mice challenged with the mutant strain manifest reduced viral load and histopathology in the liver,as well as improved survival rates.This study is of great signif-icance for deepening the understanding of the pathogenesis of FMDV and formulating prevention and con-trol measures.It also lays a solid theoretical foundation for the subsequent development of geneti-cally engineered vaccines against FMDV.关键词
O型口蹄疫病毒/反向遗传操作/3D蛋白突变/生物学特性Key words
foot-and-mouth disease virus type O/reverse genetics/3D protein mutations/biological characteristics分类
畜牧业引用本文复制引用
朱兆宇,郜原,范许许,裴丹诗,王一卓,何路,李熙忠,任青峰,郑海学,李伟伟..O型口蹄疫病毒3D突变毒株的构建及其生物学特性分析[J].中国兽医科学,2025,55(6):719-724,6.基金项目
国家自然科学基金青年项目(32202781,32302857) （32202781,32302857）
国家重点研发计划项目(2021YFD1800300) （2021YFD1800300）
国家自然科学基金重点项目(32330107) （32330107）
中国博士后科学基金项目(2023M743830) （2023M743830）
国家生猪产业技术体系项目(CARS-35,CARS-39-13) （CARS-35,CARS-39-13）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
中国农业科学院创新计划项目(CAAS-CSLPDCP-2023002,CAAS-ASTIP-2023-LVRI) （CAAS-CSLPDCP-2023002,CAAS-ASTIP-2023-LVRI）
"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2024KJ14) （2024KJ14）
兰州兽医研究所基本科研业务项目(1610312021009) （1610312021009）
