中药新药与临床药理2025，Vol.36Issue(6)：879-887,9.DOI:10.19378/j.issn.1003-9783.2025.06.005
黄花蒿水提物对青蒿素抗性鼠疟及哌喹抗性鼠疟的作用研究
Study on the Effect of Aqueous Extract of Artemisia annua L.on Artemisinin-Resistant and Piperaquine-Resistant Malaria in Mice
摘要
Abstract
Objective To evaluate the therapeutic effects of aqueous extract of Artemisia annua L.on mice infected with artemisinin-resistant and piperaquine-resistant malaria parasites from multiple perspectives.Methods Mice were divided into blank,model,positive control,and low-,medium-,and high-dose Artemisia annua L.extract groups.A murine malaria model was established by intraperitoneal injection of infected blood,and oral administration began 2 hours post-modeling and continued for 4 days.On day 4,blood smears were prepared to calculate infection rates.Liver and spleen indices were calculated,and HE staining was performed to compare histopathological changes among groups.Serum levels of tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),and interleukin-6(IL-6),as well as liver tissue levels of intercellular adhesion molecule-1(ICAM-1)and vascular cell adhesion molecule-1(VCAM-1),were measured using ELISA.Results In the efficacy experiments of Artemisia annua L.extract on artemisinin-resistant and piperaquine-resistant strains,compared with the blank group,the model group showed significantly increased infection rates,liver and spleen indices,serum TNF-α,IFN-γ,and IL-6 levels,and liver ICAM-1 and VCAM-1 levels(P＜0.01).Severe histopathological damage and significant malaria pigment deposition were observed in the liver and spleen.Compared with the model group,all treatment groups exhibited significantly reduced infection rates(P＜0.01),improved liver and spleen histopathology,and decreased malaria pigment accumulation.In the artemisinin experiment,compared with the artemisinin-resistant model group,the medium-dose Artemisia annua L.group showed a significant decrease in liver index(P＜0.05).TNF-α,IL-6,and ICAM-1 levels were significantly reduced in all Artemisia annua L.dose groups(P＜0.05,P＜0.01),IFN-γ levels were significantly reduced in the high-dose group(P＜0.05),and VCAM-1 levels were significantly reduced in the low-dose group(P＜0.05).In the piperaquine experiment,compared with the piperaquine-resistant model group,the high-dose Artemisia annua L.group showed a significant reduction in spleen index(P＜0.05).TNF-α,IFN-γ,ICAM-1,and VCAM-1 levels were significantly reduced in all Artemisia annua L.dose groups(P＜0.05,P＜0.01),and IL-6 levels were significantly reduced in the low-and high-dose groups(P＜0.05,P＜0.01).Conclusion The aqueous extract of Artemisia annua L.exhibits significant antimalarial effects against artemisinin-resistant and piperaquine-resistant murine malaria,demonstrating therapeutic potential.Its efficacy is associated with alleviating host inflammatory responses and immune reactions.关键词
黄花蒿/青蒿素/哌喹/耐药性/疟疾/炎症反应/免疫应答/小鼠Key words
Artemisia annua L./artemisinin/piperaquine/drug resistance/malaria/inflammatory response/immune reaction/mice分类
医药卫生引用本文复制引用
邱翠雯,陈培婷,余林辛,梅漫雪,邓长生..黄花蒿水提物对青蒿素抗性鼠疟及哌喹抗性鼠疟的作用研究[J].中药新药与临床药理,2025,36(6):879-887,9.基金项目
国家自然科学基金项目(82074301) （82074301）
广州市科技计划项目(202206010066). （202206010066）
