中药新药与临床药理2025，Vol.36Issue(6)：899-906,8.DOI:10.19378/j.issn.1003-9783.2025.06.007
小青龙汤配方颗粒剂及汤剂治疗变应性鼻炎小鼠的有效性与安全性比较
A Comparative Study on the Efficacy and Safety Between Dispensing Granules and Decoction Pieces of Xiaoqinglong Decoction in Treating Allergic Rhinitis in Mice
摘要
Abstract
Objective To compare the efficacy and safety of dispensing granules and decoction pieces of Xiaoqinglong Decoction(XQLD)in treating allergic rhinitis(AR)in mice.Methods Forty-two female BALB/c mice were randomly divided into a control group,a model group,a low-dose XQLD decoction pieces group(11.83 g·kg-1),a high-dose XQLD decoction pieces group(23.66 g·kg-1),a low-dose XQLD dispensing granules group(3.61 g·kg-1),a high-dose XQLD dispensing granules group(7.22 g·kg-1),and a Dexamethasone group(1 mg·kg-1),with 6 mice in each group.Mice were intraperitoneally injected with 200 µL of phosphate-buffered saline(PBS)containing 25 µg ovalbumin(OVA)and 2 mg aluminum hydroxide on days 1,6,11,and 16 to induce basic sensitization.From day 22 to day 28,mice were intranasally administered 40 µL of PBS containing 25 µg·µL-1 OVA(20 µL per nostril)once daily for one week.One hour before each intranasal administration,the treatment groups were orally administered the corresponding doses once daily for one week.Within 10 minutes after the last OVA intranasal administration,the number of sneezes and nasal scratches was recorded.HE and PAS staining were used to observe pathological changes in the nasal mucosa.ELISA was used to measure serum levels of ovalbumin-specific immunoglobulin E(OVA-sIgE),interleukin-4(IL-4),interleukin-5(IL-5),and interleukin-13(IL-13).An automatic biochemical analyzer was used to measure serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),creatinine(CREA),and urea(UREA).Results(1)Compared with the control group,the number of sneezes and nasal scratches in the model group significantly increased(P＜0.01);the nasal mucosa showed congestion,edema,disordered cell arrangement,and a significant increase in goblet cell hyperplasia(P＜0.01);serum levels of OVA-sIgE,IL-4,IL-5,and IL-13 significantly increased(P＜0.01).Compared with the model group,the number of nasal scratches in all treatment groups significantly decreased(P＜0.01),and the number of sneezes in the Dexamethasone group,high-dose XQLD dispensing granules group,and high-dose XQLD decoction pieces group significantly decreased(P＜0.05,P＜0.01);nasal mucosal congestion and edema were significantly alleviated,inflammatory cell infiltration decreased,and the number of goblet cells significantly decreased(P＜0.01);serum levels of OVA-sIgE,IL-4,IL-5,and IL-13 significantly decreased(P＜0.01).Compared with the equivalent crude drug dose decoction group,the number of nasal scratches in the low-dose XQLD dispensing granules group significantly decreased(P＜0.01);the number of goblet cells in the low-and high-dose XQLD dispensing granules groups significantly decreased(P＜0.05,P＜0.01),and serum levels of OVA-sIgE,IL-4,IL-5,and IL-13 significantly decreased(P＜0.05,P＜0.01).(2)The liver and kidney tissues of mice in all groups showed clear structures,normal hepatocyte morphology,and no abnormal changes in glomerular and tubular structures.There were no significant differences in serum levels of ALT,CREA,and UREA(P＞0.05).Compared with the control group,serum AST levels in all other groups significantly increased(P＜0.05,P＜0.01);compared with the model group,there were no significant differences in serum AST levels in all treatment groups(P＞0.05).Conclusion Both dispensing granules and docoction pieces of XQLD can effectively alleviate nasal itching symptoms,improve nasal mucosal pathological changes,and reduce serum levels of allergic inflammatory factors in AR mice.The efficacy of dispensing granules is superior to that of the equivalent crude drug dose decoction.关键词
小青龙汤/配方颗粒/饮片汤剂/变应性鼻炎/有效性/安全性/小鼠Key words
Xiaoqinglong Decoction/dispensing granules/decoction pieces/allergic rhinitis/efficacy/safety/mice分类
医药卫生引用本文复制引用
王姝玥,阮岩,孟瑜,汪咏春,骆皓然,徐玮祯,吴剑,于锋,刘元献,闫亚杰..小青龙汤配方颗粒剂及汤剂治疗变应性鼻炎小鼠的有效性与安全性比较[J].中药新药与临床药理,2025,36(6):899-906,8.基金项目
国家自然科学基金项目(82274590,82305325,82474584) （82274590,82305325,82474584）
广东省基础与应用基础研究基金项目(2023A1515220080,2021A1515110446,2024A1515011440) （2023A1515220080,2021A1515110446,2024A1515011440）
广州市科技基础与应用基础研究项目(202201011211) （202201011211）
广州市科技计划项目(202201020308,2023A03J0587) （202201020308,2023A03J0587）
深圳市自然科学基金面上项目(JCYJ20210324111205016) （JCYJ20210324111205016）
深圳三名工程"广州中医药大学第一附属医院阮岩教授中医五官科团队"(SZZYSM202311005) （SZZYSM202311005）
广州市耳鼻咽喉科临床与基础研究重点实验室(2023A03J0521). （2023A03J0521）
