加味麦门冬汤通过上调外周血和肿瘤微环境中NK和CD8+T细胞水平抑制肺癌转移
Modified Maimendong Decoction Inhibits Lung Cancer Metastasis by Up-Regulating Levels of NK and CD8+T Cells in Peripheral Blood and Tumor Microenvironment
摘要
Abstract
Objective To explore the mechanism of modified maimendong decoction(MMD)in inhibiting lung cancer metastasis from the perspective of immune regulation.Methods CTC-TJH-01 and LLC cells were intervened with different concentrations of modified maimendong decoction.The cell proliferation was detected with a CCK-8 kit,apoptosis was detected with an Annexin V-FITC/PI kit,and cell migration was detected through Transwell assays.A lung metastasis model was established through the tail vein injection of LLC cells into C57BL/6 mice,and body weight change and lung tumor metastasis in the mice were evaluated after continuous gavage intervention with MMD.HE staining,immunohistochemistry,and immunofluor-escence were employed to observe the histomorphology,Ki-67 protein level,and NK and T cell levels of metastatic lesions.The levels of NK and T cells in the peripheral blood of mice were detected throughflow cytometry.Results MMD had no significant inhibitory effect on the proliferation,apoptosis,and migration of CTC-TJH-01 and LLC cells in vitro.In mice,MMD could significantly inhibit the lung metastasis of LLC cells,increase the proportion of NK and CD8+T cells in peripheral blood and tumor microenvironment(P<0.05),and reduce the expression of Ki-67 protein in metastatic tumor tissues(P<0.05).Conclusion MMD may inhibit the growth of metastatic tumors by upregulating the expression levels of NK and CD8+T cells in peripheral blood to promote the elimination of circulating tumor cells,and regulating the infiltration of NK and CD8+T cells in the immune microenvironment of metastatic tumors,then play an antimetastatic role in lung cancer.关键词
肺癌转移/加味麦门冬汤/NK细胞/CD8+T细胞/正虚伏毒Key words
Lung cancer metastasis/Modified maimendong decoction/NK cells/CD8+T cells/Deficiency of vital qi and hidden toxin分类
临床医学引用本文复制引用
张志鹏,田建辉,阙祖俊,陈子琦,罗斌,刘诗卉..加味麦门冬汤通过上调外周血和肿瘤微环境中NK和CD8+T细胞水平抑制肺癌转移[J].肿瘤防治研究,2025,52(6):466-473,8.基金项目
National Natural Science Foundation of China(No.82174245,82104943) （No.82174245,82104943）
Shanghai Municipal Health Leading Talents Program(No.2022LJ014) （No.2022LJ014）
Future Plan for Traditional Chinese Medicine Development of Science and Technology of Shanghai Municipal Hospital of Traditional Chi-nese Medicine(No.WL-HBRC-2021 001K) （No.WL-HBRC-2021 001K）
Shanghai Traditional Chinese Medicine"Three-Year Action Plan"(No.ZY(2021-2023)-0211) （No.ZY(2021-2023）
Shanghai Frontier Research Base of Disease and Syndrome Biology of Inflammatory Cancer Transformation(No.2021KJ03-12)国家自然科学基金(82174245,82104943) （No.2021KJ03-12）
上海市卫生健康委领军人才项目(2022LJ014) （2022LJ014）
上海市中医医院未来计划科技发展基金(WL-HBRC-2021001K) （WL-HBRC-2021001K）
上海市进一步加快中医传承创新发展三年行动计划[ZY(2021-2023)-0211] （2021-2023）
上海市炎癌转化病症生物学前沿研究基地项目(2021科技03-12) （2021科技03-12）
