肿瘤防治研究2025，Vol.52Issue(6)：474-480,7.DOI:10.3971/j.issn.1000-8578.2025.24.0553
肾透明细胞癌中Cyclin F的表达及其对肾癌细胞株生物学行为的影响
Cyclin F Expression in Clear Cell Renal Cell Carcinoma and Its Effect on Biological Behavior of Renal Carcinoma Cell Lines
摘要
Abstract
Objective To investigate the expression of Cyclin F in clear cell renal cell carcinoma(ccRCC),its clinicopathological characteristics,and its effect on the biological behavior of renal cancer cell lines Methods RT-qPCR and Western blot were used to detect the mRNA and protein expression of Cyclin F in fresh ccRCC specimens.Immunohistochemistry assay was performed to detect the expression of Cyclin F protein in 80 paraffin samples.CCK-8 assay,scratch assay,and flow cytometry were conducted to determine the effects of Cyclin F overexpression on the proliferation,migration,and apoptosis of renal cancer cell lines.Results The expression of Cyclin F in cancer tissues was higher than that in adjacent tissues at the mRNA level(P<0.0285).The expression of Cyclin Fprotein in cancer tissues was lower than that in adjacent tissues(P<0.001).The analysis of clinicopathological parameters showed that the low expression of Cyclin F was correlated with WHO/ISUP grade,Ki67 index,and age(P=0.041,0.047,0.008,).In vitro experiments confirmed that overexpression of Cyclin F promoted the proliferation(P<0.001)and migratory ability(24 h P=0.003,48 h P=0.0058)of the RCC 786-O cell line,while inhibiting apoptosis(P=0.0019).Conclusion The low expression of Cyclin F protein in ccRCC tissuesis associated with high ISUP grade,high Ki67 index,oldage,and prognosis of patients.关键词
细胞周期蛋白F/肾透明细胞癌/细胞周期蛋白/临床病理/生物学行为Key words
Cyclin F/Clear cell renal cell carcinoma/Cyclins/Clinicopathology/Biological behavior分类
临床医学引用本文复制引用
苏敏,王燕,华结,王天云,徐胜男,奎翔..肾透明细胞癌中Cyclin F的表达及其对肾癌细胞株生物学行为的影响[J].肿瘤防治研究,2025,52(6):474-480,7.基金项目
The Second Affiliated Hospital of Kunming Medical University Hospital Science and Technology Program(No.XJ2021005501) （No.XJ2021005501）
The Second Affiliated Hospital of Kunming Medical University"Talent Echelon Development"Training Program(No.RCTDXK-202301) 昆明医科大学第二附属医院院内科技计划项目(No.XJ2021005501) （No.RCTDXK-202301）
昆明医科大学第二附属医院"人才梯队建设"培养项目(No.RCTDXK-202301) （No.RCTDXK-202301）
