中国当代儿科杂志2025，Vol.27Issue(6)：716-722,7.DOI:10.7499/j.issn.1008-8830.2411112
基于液相色谱质谱法对儿童支气管哮喘脂质分析的前瞻性研究
Lipid analysis in children with bronchial asthma based on liquid chromatography-mass spectrometry:a prospective study
冯特 1谢利娜 1张玉会 1郭燕军1
作者信息
- 1. 郑州大学附属儿童医院呼吸科二病区,河南 郑州 450018
- 折叠
摘要
Abstract
Objective To explore the lipidomic characteristics of children with bronchial asthma(hereafter referred to as asthma)and identify potential biomarkers for asthma.Methods A total of 26 asthmatic children were prospectively enrolled as the asthma group,and 20 healthy children served as the healthy control group.The asthma group was further divided into atopic(n=13)and non-atopic(n=13)subgroups based on IgE levels.Serum lipid metabolites were analyzed using liquid chromatography-mass spectrometry,followed by statistical analysis and data visualization.Results A total of 1 435 lipids were detected in the 46 children,primarily glycerophospholipids(625/1 435,43.55%).Significant differences were observed in serum lipid profiles between the asthma and control groups.Twelve significantly differential lipids were identified,with receiver operating characteristic curve analysis showing that phosphatidylserine(PS)(18:0/20:4)and ceramide(Cer)(c16:0)exhibited the highest diagnostic value for asthma.The relative abundances of PS(18:0/20:4)and PS(18:0/22:6)were higher in the atopic subgroup than in the non-atopic subgroup(P<0.05)and positively correlated with total IgE levels in asthmatic children(r=0.675 and 0.740,respectively;P<0.05).Conclusions Asthmatic children exhibit significant lipid metabolic disturbances,primarily characterized by abnormal glycerophospholipid metabolism.Among these,PS(18:0/20:4)and Cer(c16:0)demonstrate specific alterations and may serve as potential diagnostic biomarkers for asthma.Furthermore,the positive correlation between PS(18:0/20:4)and PS(18:0/22:6)levels and serum total IgE suggests their possible involvement in immune regulation in asthma.关键词
支气管哮喘/生物标志物/脂质组学/儿童Key words
Bronchial asthma/Biomarker/Lipidomics/Child引用本文复制引用
冯特,谢利娜,张玉会,郭燕军..基于液相色谱质谱法对儿童支气管哮喘脂质分析的前瞻性研究[J].中国当代儿科杂志,2025,27(6):716-722,7.
