安徽农业科学2025，Vol.53Issue(12)：141-144,148,5.DOI:10.3969/j.issn.0517-6611.2025.12.032
6种病毒复合侵染"玛莎莉"甘薯的鉴定和分析
Identification and Analysis of a Mixed Infection of Six Viruses in Sweet Potato Cultivar"Mashali"
摘要
Abstract
Transcriptome sequencing and RT-PCR were performed on four sweet potato samples collected from Linyi City,Shandong Prov-ince,to study the types of viruses that infected sweet potato"Mashali",in order to provid references for the breeding of virus-free sweet potato seedlings and the formulation of prevention and control strategies.The sweet potato samples of"Mashali"showed obvious mosaic and crinkling symptoms.Six known viruses,including sweet potato feathery mottle virus(SPFMV),sweet potato laten virus(SPLV),sweet potato leaf curl virus(SPLCV),sweet potato virus G(SPVG),sweet potato virus C(SPVC)and sweet potato virus 2(SPV2)were identified.The specific primers of the above viruses were selected for RT-PCR detection,the target bands with the expected size of the six viruses were obtained,and the target virus sequences were verified by sequencing.The results of RT-PCR and transcriptome sequencing were consistent with the results of 3 samples containing 6 viruses and 1 sample containing 4 viruses.The phylogenetic analysis based on the CP gene showed that the detected SPFMV had a close evolutional relationship with SPFMV russet crack(RC),the detected SPLCV,SPV2 and SPVC were most closely related to the isolates of China,SPVG was most closely related to the isolates of CH1,and there were significant differences among isolates from SPLV collected from different region.The results of this study will provide theoretical basis for the establishment of field control and virus-free tech-nology system of sweet potato cultivar"Marasali".关键词
甘薯病毒病/复合侵染/转录组/RT-PCRKey words
Sweet potato virus disease/Mixed infection/Transcriptome/RT-PCR分类
农业科技引用本文复制引用
张晓艳,刘玉珊,朱文莉,唐洪杰,袁鑫,闫煜鑫,孙杰..6种病毒复合侵染"玛莎莉"甘薯的鉴定和分析[J].安徽农业科学,2025,53(12):141-144,148,5.基金项目
山东省自然科学基金项目(ZR2022QC033) （ZR2022QC033）
浙江大学山东(临沂)现代农业研究院院长基金项目(AZJU-2022-DF01001). （临沂）
