安徽医科大学学报2025，Vol.60Issue(6)：1000-1008,9.DOI:10.19405/j.cnki.issn1000-1492.2025.06.004
沉默circFADS2通过miR-152-3p/SLC7A11信号轴诱导结直肠癌细胞铁死亡
Silencing circFADS2 induces ferroptosis in colorectal cancer cells through miR-152-3p/SLC7A11 signaling axis
摘要
Abstract
Objective To investigate the effect and mechanism of silencing circFADS2 on ferroptosis in colorectal cancer(CRC)cells.Methods The human colon adenocarcinoma cell line SW480 was used as the research ob-ject.circFADS2 siRNA interference plasmid(si-circFADS2)and its negative control si-NC,miR-152-3p inhibitor and its negative control inhibitor-NC,miR-152-3p mimics and its negative control mimics NC,SLC7A11 overex-pression plasmid(oe-SLC7A11)and its negative control vector were transfected into SW480 cells.Methylthiazolyl-diphenyl-tetrazolium bromide(MTT)method was used to detect cell proliferation activity;The contents of ferrous ion(Fe2+),malondialdehyde(MDA)and the activities of superoxide dismutase(SOD)and glutathione(GSH)in cells were detected by chemical method.Dichlorodihydrofluorescein diacetate(DCFH-DA)probe was used to detect the level of reactive oxygen species(ROS)in cells.Quantitative Real-time polymerase chain reaction(qPCR)was used to detect the expression levels of miR-152-3p and SLC7A11 mRNA in cells.Western blot was used to detect the expression levels of ferroptosis-related proteins such as SLC7A11 and GPX4 in cells.Dual lucif-erase reporter gene experiment was used to verify the sponge adsorption relationship between miR-152-3p and circFADS2,and the targeted regulation relationship between miR-152-3p and SLC7A11.Results Compared with blank group,the proliferation activity of si-circFADS2 group decreased,the levels of Fe2+,MDA and ROS in-creased,and the levels of SOD and GSH decreased;At the same time,the expression level of miR-152-3p in-creased,and the protein expression levels of SLC7A11 and GPX4 decreased in cells(all P＜0.05).Compared with si-circFADS2 group,the proliferation activity of si-circFADS2+inhibitor group increased,the levels of Fe2+,MDA and ROS in cells decreased,and the levels of SOD and GSH increased;At the same time,the expression level of miR-152-3p decreased,and the protein expression levels of SLC7A11 and GPX4 increased(all P＜0.05).The results of dual luciferase reporter gene experiment showed that SLC7A11 was a downstream target gene of miR-152-3p.Conclusion Silencing circFADS2 can induce ferroptosis in CRC cells possibly by targeting the miR-152-3p/SLC7A11 signaling axis.关键词
circFADS2/结直肠癌/SW480细胞/铁死亡/miR-152-3p/溶质载体家族7成员11Key words
circFADS2/colorectal cancer/SW480 cells/death of iron/miR-152-3p/solute carrier family 7 member 11分类
医药卫生引用本文复制引用
蒋良君,卢先州..沉默circFADS2通过miR-152-3p/SLC7A11信号轴诱导结直肠癌细胞铁死亡[J].安徽医科大学学报,2025,60(6):1000-1008,9.基金项目
湖南省科技创新计划社会化出资项目(编号:2020SKC2009) （编号:2020SKC2009）
湖南省自然科学基金(编号:2024JJ7461) （编号:2024JJ7461）
湖南省卫生健康委科研计划项目(编号:D202303038378) （编号:D202303038378）
衡阳市科技局指导性计划(编号:202222035832) Hunan Province Socialized Funding Projects for Science and Technology Innovation Plan(No.2020SKC2009) （编号:202222035832）
Natural Science Foundation of Hunan Province(No.2024JJ7461) （No.2024JJ7461）
General Guidance Project of Hubei Provincial Health Commission(No.D202303038378) （No.D202303038378）
Guiding Plan Project of Science and Technology Bu-reau of Hengyang(No.202222035832) （No.202222035832）
