安徽医科大学学报2025，Vol.60Issue(6)：1022-1029,8.DOI:10.19405/j.cnki.issn1000-1492.2025.06.007
miR-224-5p对人肝癌细胞HepG2增殖、凋亡、侵袭及迁移的作用机制
The mechanism of miR-224-5p on proliferation,apoptosis,invasion,and migration of human hepatoma cells HepG2
摘要
Abstract
Objective To investigate the mechanism of miR-224-5p on proliferation,apoptosis,invasion and mi-gration of human hepatocellular carcinoma HepG2 cells.Methods The RNA expression levels of miR-224-5p and early growth responsive gene 2(EGR2)in patients with hepatocellular carcinoma were obtained from the TCGA dataset.Normal human hepatocytes LO2 and hepatoma cells HepG2 were cultured in vitro,and the HepG2 cells were transfected with lentiviral vectors(knockdown of miR-224-5p),small interfering RNA fragments or overex-pression vectors(interference and overexpression of EGR2).The expression levels of miR-224-5p and EGR2 in hepatocellular carcinoma cDNA chips and cells were detected by quantitative real-time PCR(qPCR).The expres-sion level of EGR2 protein was detected by Western blot.Dual luciferase reporter gene assay was used to detect the binding of miR-224-5p to EGR2.HepG2 cells positive rate were detected by EdU assay,apoptosis rate was detec-ted by flow cytometry,cell invasion number was detected by Transwell assay,and cell mobility was detected by scratch assay.Results Compared with paracancerous tissues,the expression of miR-224-5p was increased and the expression of EGR2 mRNA decreased in HCC tissues.Compared with LO2 group,the expression of miR-224-5p in HepG2 cells increased,and the expression of EGR2 mRNA and protein decreased.Compared with the Lv-sh-NC group,the 24 h EdU positive cell rate,cell invasion number,and 48 h cell mobility of HepG2 cells in the Lv-sh-miR-224-5p group decreased,while the apoptosis rate increased.Compared with Oe-NC group,24 h EdU positive cell rate,cell invasion number,and 48 h cell mobility of HepG2 cells in Oe-EGR2 group decreased,while apopto-sis rate increased.Compared with Lv-sh-NC group,the expression of EGR2 protein in Lv-sh-miR-224-5p group in-creased.Compared with Lv-sh-miR-224-5p+si-NC group,24 h EdU positive cell rate,cell invasion rate,and 48 h cell mobility of HepG2 cells in Lv-sh-miR-224-5p+si-EGR2 group increased,while apoptosis number de-creased.Conclusion miR-224-5p can promote proliferation,invasion,and migration of HepG2 cells and inhibit apoptosis via binding with EGR2.关键词
miR-224-5p/HepG2/增殖/凋亡/侵袭及迁移/早期生长反应因子2Key words
miR-224-5p/HepG2/proliferation/apoptosis/invasion and migration/early growth responsive gene 2分类
临床医学引用本文复制引用
顾铃毓,王利新,崔洁,董辉..miR-224-5p对人肝癌细胞HepG2增殖、凋亡、侵袭及迁移的作用机制[J].安徽医科大学学报,2025,60(6):1022-1029,8.基金项目
国家自然科学基金(编号:82002955) （编号:82002955）
宁夏自然科学基金(编号:2022AAC03567) National Natural Science Foundation of China(No.82002955) （编号:2022AAC03567）
Natural Science Foundation of Ningxia Hui Autonomous Region(No.2022AAC03567) （No.2022AAC03567）
