安徽医科大学学报2025，Vol.60Issue(6)：1030-1035,6.DOI:10.19405/j.cnki.issn1000-1492.2025.06.008
不同剂量万古霉素诱导大鼠急性肾损伤模型的评价
Evaluation of acute kidney injury model in rats induced by different doses of vancomycin
摘要
Abstract
Objective To investigate the method and evaluation of using different doses of vancomycin(VCM)to construct an acute kidney injury(AKI)model in SD rats.Methods The 6-week-old male SD rats were randomly divided into 4 groups:normal control(NC)group,VCM(200,400,600)mg/kg group.The drug was given by intraperitoneal injection once a day for 7 days.The levels of blood urea nitrogen(BUN)and creatinine(Cre)were detected.The kidney was weighed and the kidney body ratio was calculated.Renal tissue was stained with HE and PAS,and the pathological injury of kidney was observed under light microscope.Results With the increase of VCM dose,serum BUN and Cre levels in each dose group showed an upward trend(F=3.85,P＜0.05;F=9.02,P＜0.01).Compared with the VCM(200,400)mg/kg groups,the Cre level in the VCM 600 mg/kg group was the highest,with statistically significant differences(P＜0.01,P＜0.05).As the VCM dose in-creased,body weight of rats in each dose group decreased(F=54.81,P＜0.01),while total kidney weight and kidney body ratio increased(F=11.13,F=14.43,both P＜0.01).Significant statistically differences(P＜0.01,P＜0.05)were observed in body weight,total kidney weight and kidney body ratio between the VCM 600 mg/kg group and the VCM(200,400)mg/kg groups.HE staining and PAS staining showed that renal tubules in each dose group were damaged to varying degrees.With the increase of VCM dose,the pathological score of kidney injury increased(F=105.80,F=28.89,both P＜0.01),and the pathological damage of renal tubular epithelial cells such as shedding,swelling and cast formation was the most serious in VCM 600 mg/kg group.Conclusion The AKI model in rats can be stably established by intraperitoneal injection of VCM at 600 mg/kg once a day for 7 days.关键词
急性肾损伤/动物模型/肾毒性/万古霉素/剂量/大鼠Key words
acute kidney injury/animal model/nephrotoxicity/vancomycin/dose/rat分类
药学引用本文复制引用
马菲,邓琴香,施瑞,王春,徐丙发..不同剂量万古霉素诱导大鼠急性肾损伤模型的评价[J].安徽医科大学学报,2025,60(6):1030-1035,6.基金项目
安徽省重点研究与开发计划(编号:2023s07020003) （编号:2023s07020003）
安徽医科大学第三附属医院基础与临床合作研究提升计划培育专项(编号:2022sfy016) Key Research and Development Program of Anhui Province(No.2023s07020003) （编号:2022sfy016）
Special Cultivation Project for the Enhancement Plan of Basic and Clinical Collaborative Research of the Third Affiliated Hospital of Anhui Medical University(No.2022sfy016) （No.2022sfy016）
