安徽医科大学学报2025，Vol.60Issue(6)：1043-1051,9.DOI:10.19405/j.cnki.issn1000-1492.2025.06.010
环状RNA hsa_circ_0002938在胃癌的表达及功能
The expression and function of circular RNA hsa_circ_0002938 in gastric cancer
摘要
Abstract
Objective To investigate the expression and biological function of circular RNA(circRNA)hsa_circ_0002938 in gastric cancer.Methods 45 pairs of tumor tissues and para-cancerous tissues from gastric cancer pa-tients who underwent tumor reduction surgery at the hospital were collected.Human gastric cancer cells SGC-7901,BGC-823,HGC-27,MGC-803 and immortalized gastric mucosal epithelial cells GES-1 were routinely cultured.RT-qPCR was utilized to detect the expression of hsa_circ_0002938 in gastric cancer tissues and cells.Chi-square test was used to analyze its association with the clinicopathological characteristics of patients,and the Kaplan-Meier method was employed to assess the relationship between hsa_circ_0002938 expression levels and patient prognosis.The effects of hsa_circ_0002938 on the proliferation,migration and invasion abilities of gastric cancer cells were detected by CCK-8,scratch wound healing and Transwell assays.Bioinformatics was used to predict the miRNA and its downstream target genes that hsa_circ_0002938 might bind to.The competitive endogenous RNA regulatory network was constructed and the functions of the target genes were enriched.Results The expression of hsa_circ_0002938 in gastric cancer tissues and cells was much higher than that in para-cancerous tissues and GES-1 cells(P＜0.05).By contrast,patients in the high hsa_circ_0002938 expression group had significantly shorter 2-year pro-gression-free survival than that in the low expression group after surgery(P＜0.01).Moreover,knocking down the expression of hsa_circ_0002938 reduced the proliferative,migratory,and invasive abilities of gastric cancer cells(P＜0.05).Bioinformatics predictive analysis showed that hsa_circ_0002938 could bind to hsa-miR-342-3p and hsa-miR-503-5p.The downstream target genes of miRNA were involved in several cancer-related pathways,like mitogen-activated protein kinase(MAPK),hippo and Wnt signaling pathway.Conclusion Hsa_circ_0002938,which is highly expressed in gastric cancer tissues and cells,is closely associated with poor patient prognosis.Knockdown of hsa_circ_0002938 inhibits the proliferation,migration,and invasion abilities of gastric cancer cells.The study reveals the potential role of hsa_circ_0002938 in the progression of gastric cancer,offering new insights into its prevention and treatment.关键词
胃癌/hsa_circ_0002938/SGC-7901细胞/BGC-823细胞/增殖/迁移/侵袭Key words
gastric cancer/hsa_circ_0002938/SGC-7901 cell/BGC-823 cell/proliferation/migration/invasion分类
临床医学引用本文复制引用
李晓鹏,刘思涵,赵一杰,李书梅,殷小磊..环状RNA hsa_circ_0002938在胃癌的表达及功能[J].安徽医科大学学报,2025,60(6):1043-1051,9.基金项目
河北省医学科学研究重点课题(编号:20230820) Key Project of Hebei Provincial Medical Science Research(No.20230820) （编号:20230820）
