安徽医科大学学报2025，Vol.60Issue(6)：1068-1073,6.DOI:10.19405/j.cnki.issn1000-1492.2025.06.013
ATF3与Smad4在翼状胬肉中的表达及相互作用
The expression and interaction of ATF3 and Smad4 in pterygium
摘要
Abstract
Objective To investigate the expression and interaction of activating transcription factor 3(ATF3)and smad family member 4(Smad4)in pterygium.Methods In this study,two sets of data GSE51995 and GSE2513 in NCBI database were analyzed by bioinformatics methods to screen out the key difference-expressed gene ATF3 in pterygium when normal conjunctiva was used as the control.The differential genes identified by bioinformatics anal-ysis were further detected by reverse transcription quantitative polymerase chain reaction(RT-qPCR).Finally,the literatures were searched and The Gene Transcription Regulation Database(GTRD)was used to predict whether there was a target gene Smad4 that might bind to the target gene.The expression of Smad4 in normal conjunctival and pterygium tissues was verified by RT-qPCR,and the interaction between the target protein ATF3 and Smad4 in the pterygium was investigated through Co-Immunoprecipitation(Co-IP).Results The results of bioinformatics a-nalysis showed that compared with normal conjunctival tissue,there was significantly different expression of ATF3 gene in pterygium tissue(P＜0.05).RT-qPCR confirmed that ATF3 was less expressed in pterygium tissue than normal conjunctival tissue(P＜0.001).Genomic data from the GTRD database revealed that Smad4 contains two ATF3 binding motifs,suggesting functional interaction potential.Compared with normal conjunctiva,RT-qPCR re-vealed Smad4 downregulation in pterygium tissues(P＜0.01);Co-IP demonstrated enhanced interaction between Smad4 and ATF3 in pterygium tissues following immunoprecipitation with anti-ATF3 antibodies(P＜0.05).Con-clusion ATF3 interacts with Smad4 in pterygium,and the low expression of both in pterygium tissues and their in-teraction may be associated with the pathogenesis of pterygium.关键词
翼状胬肉/ATF3/Smad4/相互作用/初步探究Key words
pterygium/ATF3/Smad4/interaction/preliminary investigation分类
临床医学引用本文复制引用
华耘,韩欢欢,陈冬梅,刘佳琳,赵新荣..ATF3与Smad4在翼状胬肉中的表达及相互作用[J].安徽医科大学学报,2025,60(6):1068-1073,6.基金项目
国家自然科学基金(编号:82060171) National Natural Science Foundation of China(No.82060171) （编号:82060171）
