安徽医科大学学报2025，Vol.60Issue(6)：1080-1085,6.DOI:10.19405/j.cnki.issn1000-1492.2025.06.015
宫颈组织和细胞中BRD4在HPV16病毒复制中的作用
The role of BRD4 in HPV16 virus replication in cervical tissue and cells
摘要
Abstract
Objective To explore the relationship between the replication-associated bromodomain protein 4 (BRD4)and human papillomavirus(HPV)16(HPV16)viral load in cervical squamous cell carcinoma and CIN Ⅰ tissues,confirm the effects of BRD4 degradation agent MZ1 on viral load.Methods Thirty HPV 16-positive cervi-cal cancer specimens and 30 non-cervical cancer specimens were collected,and the viral load of the samples was detected by real-time fluorescence quantitative PCR,and the expression of BRD4 was analyzed by immunohisto-chemistry and Western blot.Results The viral load was higher in the samples of cervical cancer group than in the samples of non-cancer group,the difference is statistically significant(P＜0.01).Immunohistochemistry results showed that the expression of BRD4 were significantly higher in cervical cancer specimens than in noncancerous specimens,the difference is statistically significant(P＜0.05).BRD4 expression was significantly and positively correlated with high viral loads,the difference is statistically significant(P＜0.001).the BRD4 degradation agent MZ1 significantly reduced the viral load,the difference is statistically significant(P＜0.01).Conclusion BRD4 may be involved in the replication of HPV16 virus,and BRD4 degradation agent MZ1 can inhibit the replication of HPV16 virus.关键词
宫颈癌/溴结构域蛋白4/人乳头瘤病毒16型/病毒载量/MZ1Key words
cervical cancer/bromodomain protein 4/human papillomavirus16/viral load/MZ1分类
基础医学引用本文复制引用
王乐,李伟欣,董杨柳,赵先,朱鑫丽,张雪晨,者湘漪,潘泽民..宫颈组织和细胞中BRD4在HPV16病毒复制中的作用[J].安徽医科大学学报,2025,60(6):1080-1085,6.基金项目
国家自然科学基金(编号:82060518、U1503125) （编号:82060518、U1503125）
新疆生产建设兵团科技发展专项(编号:2019BC007) （编号:2019BC007）
石河子大学自主资助支持校级科研项目(编号:ZZZC202134) National Natural Science Foundation Project(Nos.82060518,U1503125) （编号:ZZZC202134）
Special Program for Scientific and Technological Development of the Xinjiang Production and Construction Corps(No.2019BC007) （No.2019BC007）
Shihezi University Self-Funded Support Project(No.ZZZC202134) （No.ZZZC202134）
