安徽医科大学学报2025，Vol.60Issue(6)：1086-1090,5.DOI:10.19405/j.cnki.issn1000-1492.2025.06.016
内质网应激在高脂诱导的代谢障碍相关脂肪变性肝病中的作用
Endoplasmic reticulum stress involved in high-fat induced metabolic dysfunction-associated steatotic liver disease
摘要
Abstract
Objective To investigate the role of endoplasmic reticulum stress in the occurrence and development of fatty liver induced by high fat.Methods In the high-fat Drosophila model,the high-fat group was fed with high-fat medium,while the control group was fed with normal medium;in the mouse fatty liver model,the high-fat group was fed with high-fat diet,and the control group was fed with normal diet;in the HepG2 cell steatosis model,the high-fat group was induced by palmitic acid(PA),and the control group was cultured with DMEM.The fat body size of the third instar larvae of Drosophila melanogaster was photographed.Steatosis in mice liver and HepG2 cells was observed by H&E and Oil Red staining.The expression levels of ATF6,Bip and CHOP in the third instar lar-vae,liver tissues of mice and HepG2 cells were analyzed by quantitative real-time polymerase chain reaction(qPCR)and Western blot.Results In Drosophila model,fat body and fat storage were obviously increased in high fat fed flies when compared with control group.The formation of liver fat droplets and cells vacuolation were confirmed by H&E and Oil Red staining in mice livers fed with high fat and HepG2 cells with palmitic acid treat-ment.The expression levels of ATF6,Bip and CHOP were significantly increased in third instar larvae and mice livers fed with high fat and palmitic acid treated HepG2 cells with palmitic acid treatment.Conclusion High fat may induce the occurrence and development of hepatic steatosis by activating endoplasmic reticulum stress.关键词
内质网应激/高脂/脂肪肝/果蝇/棕榈酸/脂肪变性Key words
endoplasmic reticulum stress/high fat/MASLD/Drosophila/palmitic acid/seatosis分类
生物学引用本文复制引用
胡韦康,周文静,张军,杨振,白红枚,王子涵,齐胤良,张胜权,张素梅..内质网应激在高脂诱导的代谢障碍相关脂肪变性肝病中的作用[J].安徽医科大学学报,2025,60(6):1086-1090,5.基金项目
安徽省高校自然科学研究项目(编号:2023AH050564) Natural Science Research Project of Anhui Educational Committee(No.2023AH050564) （编号:2023AH050564）
