生物资源2025，Vol.47Issue(3)：259-269,11.DOI:10.14188/j.ajsh.20250315001
基于SSR和ISSR标记的鱼腥草遗传多样性分析
Genetic diversity analysis of Houttuynia cordata Thunb.based on SSR and ISSR markers
摘要
Abstract
Houttuynia cordata is an important medicinal and edible plant with abundant germplasm resources.However,systematic studies on its genetic diversity have been relatively scarce in the past five years,particularly those utilizing SSR molecular markers,which has hindered progress in variety improvement and resource conservation.To ad-dress this issue,this study employed both SSR and ISSR markers to analyze the genetic diversity of 140 H.cordata sam-ples collected from 10 geographic populations across eight provincial-level administrative regions in China.The results re-vealed a high proportion of polymorphic loci,with SSR and ISSR markers showing rates of 94.20%and 93.20%,re-spectively,indicating high genetic diversity in H.cordata.Genetic differentiation and gene flow analysis demonstrated that the majority of genetic variation existed among populations,with genetic differentiation coefficients(GST)of 0.9508(SSR)and 0.8903(ISSR)and low number of migrants(Nm),suggesting that geographic isolation may be the primary driver of population differentiation.Cluster analysis and principal component analysis(PCA)further revealed close genetic relationships between certain populations(e.g.,Mianyang and Chengdu in Sichuan;Maoming in Guang-dong and Liuzhi in Guizhou),while the genetic distances of other populations showed no obvious correlation with geo-graphic distribution.These findings provide valuable insights for the conservation and breeding of H.cordata germplasm resources.关键词
鱼腥草/简单序列重复/简单重复序列间区/遗传多样性Key words
Houttuynia cordata/simple sequence repeats(SSR)/inter-simple sequence repeat(ISSR)/genetic diversity分类
医药卫生引用本文复制引用
肖文韬,胡中立,王彬,杨赛,董先婷,刘燕利,鄢姗,吴方华,严守雷,刁英..基于SSR和ISSR标记的鱼腥草遗传多样性分析[J].生物资源,2025,47(3):259-269,11.基金项目
武汉轻工大学人才引进科研启动基金(2021RZ099) （2021RZ099）
