北京中医药2025，Vol.44Issue(5)：548-556,9.DOI:10.16025/j.1674-1307.2025.05.003
基于AGEs-bFGF交联通路探讨复方黄柏液涂剂外敷促进糖尿病足溃疡大鼠创面愈合的作用机制
Mechanism of Compound Huangbai Liquid liniment in promoting wound tissue healing in diabetic foot ulcer model rats based on AGEs-bFGF cross-linking pathway
摘要
Abstract
Objective To investigate the mechanism by which Compound Huangbai Liquid promotes the healing of diabetic foot ulcer(DFU)wounds in rats based on the advanced glycation end products(AGEs)/basic fibroblast growth factor(bFGF)signaling pathway.Methods SPF-grade male rats were randomly divided using a random number table into five groups:blank group,model group,growth factor group,Compound Huangbai Liquid group,and growth factor+Compound Huangbai Liquid group.Except for the blank group,all rats were fed a high-sugar,high-fat diet and received an intraperitoneal injection of streptozotocin(STZ)to establish diabetes and DFU models.The corresponding treatments were administered,and tissue samples were collected from 80 rats.Wound healing rates were compared on days 7 and 14 after DFU modeling.Histopathological changes in wound tissues were observed using hematoxylin-eosin(HE)and Masson staining.Immunofluorescence was used to detect the expression of bFGF,fibroblast growth factor receptor(FGFR),and proliferating cell nuclear antigen(PCNA)in wound tissues.Enzyme-linked immunosorbent assay(ELISA)was employed to measure the levels of vascular endothelial growth factor(VEGF),bFGF,and factors related to the AGEs-RAGE signaling pathway in wound skin tissue.Results On days 7 and 14,compared with the blank group,the model group showed significantly lower wound healing rates(P<0.05).Compared with the model group,the Compound Huangbai Liquid group,growth factor group,and combination group all exhibited significantly higher wound healing rates(P<0.05).ELISA results showed that levels of AGEs and RAGE in wound skin tissue were significantly decreased(P<0.05),while VEGF and bFGF levels were significantly increased(P<0.05).Immunofluorescence revealed significantly increased expression of bFGF,FGFR,and PCNA in all treatment groups(P<0.05).Histological analysis showed that all treatment groups promoted new epidermis formation and collagen fiber deposition,reduced inflammatory cell infiltration,and accelerated ulcer healing.Conclusion Compound Huangbai Liquid can promote angiogenesis and accelerate wound repair and healing in DFU rats by inhibiting the AGEs-bFGF crosslinking pathway and regulating the expression of VEGF,bFGF,PCNA,and FGFR in the wound.关键词
糖尿病足溃疡/复方黄柏液/晚期糖基化终产物/碱性成纤维细胞生长因子/信号通路/创面愈合/生长因子/大鼠Key words
diabetic foot ulcer/Compound Huangbai Liquid/AGEs/bFGF/signaling pathway/wound healing/growth factor/rat引用本文复制引用
赛序林,李友山,杨剑峰,张艳,张锦楠,余清璇,张力睿..基于AGEs-bFGF交联通路探讨复方黄柏液涂剂外敷促进糖尿病足溃疡大鼠创面愈合的作用机制[J].北京中医药,2025,44(5):548-556,9.基金项目
国家自然科学基金面上项目(81973849-H2709) （81973849-H2709）
