茶叶学报2025，Vol.66Issue(2)：1-11,11.DOI:10.20045/j.cnki.issn.2096-0220.2025.02.001
茶树光捕获叶绿素a/b结合蛋白基因CsLhca4克隆及其表达分析
Cloning and Expression of CsLhca4 in Tea Plants
摘要
Abstract
[Objective]Genes associated with the photosynthesis-promoting,light harvesting chlorophyll a/b binding protein(Lhc)in tea plants were cloned to study their functions and expressions under stress.[Method]CsLhca4s,the genes related to Lhc and their promoters in'Shuchazao'and'Longjing 43'tea cultivars were cloned.A bioinformatic analysis combined with transcriptome data was conducted to determine the expression pattern of the genes under stress and their upstream promoter cis-acting elements.Functions of the genes were analyzed.[Result]CsLhca4s were cloned from the tea cultivars.They were stable,hydrophobic proteins encoding 252 amino acids at a length of 759 bp.With a similarity of 99.6%,the two proteins differed only on the 5th codon of Shuchazao that encoded alanine(Ala)and threonine(Thr)of'Longjing 43'.Localized in chloroplasts,CsLhca4s had a secondary structure containing mainly alpha helixes and random coils.The transcriptomes of the genes showed high expression in the leaves followed by the stems but low in the flowers and fruits,and almost none in the roots.The gene expressions were affected by light exposure that declined in 4-8h under shading.They also responded to stress under drought,salt or MEJA treatment.The CsLhca4 promoters contained cis-acting elements involved in the responses to light and hormones as well as the regulation of cellular cycles.[Conclusion]The CsLhca4s of'Shuchazao'and'Longjing 43'tea cultivars were tissue-specific that basically expressed in photosynthetic organs.They responded to drought and salt stresses,thus could be a candidate for application in breeding resistant varieties.关键词
茶树/CsLhca4基因/光捕获叶绿素a/b结合蛋白/表达分析Key words
Camellia sinensis/CsLhca4/light-harvesting chlorophyll a/b-binding protein/expression analysis分类
农业科学引用本文复制引用
张佳琪,庄静,罗微,蒋珊,高艺涵,胡志航,杨妮,谭杉杉,熊爱生,刘慧..茶树光捕获叶绿素a/b结合蛋白基因CsLhca4克隆及其表达分析[J].茶叶学报,2025,66(2):1-11,11.基金项目
江苏省高校优势学科项目[PAPD]. （）
