茶叶学报2025，Vol.66Issue(2)：44-49,6.DOI:10.20045/j.cnki.issn.2096-0220.2025.02.006
金龟子绿僵菌Ma54颗粒剂载体与促活剂的筛选及评价
Carrier and Activator for Making Metarhizium anisopliae Ma54 Biocontrol Granules
摘要
Abstract
[Objective]Proper carrier and activator for formulating a Metarhizium anisopliae Ma54-based biocontrol agent against Ectropis grisescens on tea bushes were investigated.[Method]Suspension of M.anisopliae Ma54 spores was mixed with kaolin,diatomite,rapeseed meal,perlite,or activated clay.Spore viability and germination rate of the mixtures after storage was measured by colony count on PDA plate observed under a microscope.Trehalose or tyrosine at 6 gradients of concentration,i.e.,0.01%,0.10%,0.20%,0.30%,0.40%,and 0.50%,were added to the PDA as an activator to examine the effect in promoting spore germination.The finalized mixture was extruded to form granules with Ma54 spore survival rate determined.[Result]None of the 5 tested carriers exerted significant effect on the spore germination.Nonetheless,perlite and kaolin extended the granule shelf life with a spore germination rate of 61.47%and 62.87%,respectively,after a 28d storage.Those rates were significantly higher than 21.07%of the counterparts without the carriers.The presence of either trehalose or tyrosine in the granules significantly increased the spore germination rate by 20.84%over control.Addition of either kaolin or perlite significantly extended the shelf life of the granules.However,the former caused 70.18%reduction on the spore vitality by the granulation process,whereas the latter induced merely a loss of 21.37%.[Conclusion]Overall,perlite was considered the preferred carrier and tyrosine the choice activator for making Ma54 spore granules as a biocontrol agent.关键词
金龟子绿僵菌/颗粒剂/载体/促活剂Key words
Metarhizium anisopliae/granule/carrier/activator分类
农业科学引用本文复制引用
李良德,李慧玲,王定锋..金龟子绿僵菌Ma54颗粒剂载体与促活剂的筛选及评价[J].茶叶学报,2025,66(2):44-49,6.基金项目
福建省属公益类科研院所基本科研项目—自主类项目(2021R1029009、2023R1088) （2021R1029009、2023R1088）
福建省科技重大专项(2024NZ029030) （2024NZ029030）
福建省农业科学院青年科技英才项目(YC2021008). （YC2021008）
